多重耐药因子A在临床菌株中的检测与分析

doi:10.3969/j.issn.1673-8640.2013.04.001

检验医学 ›› 2013, Vol. 28 ›› Issue (4): 259-262.DOI: 10.3969/j.issn.1673-8640.2013.04.001

• 临床应用研究.论著 • 下一篇

多重耐药因子A在临床菌株中的检测与分析

李俐¹,蒋燕群²,沈建雄¹,薛志忠¹

1. 上海中医药大学附属岳阳中西医结合医院检验科，上海 200437；2. 上海交通大学附属第六人民医院检验科，上海 200233

收稿日期:2012-04-02 修回日期:2012-06-18 出版日期:2013-04-30 发布日期:2013-04-05
通讯作者: 薛志忠，联系电话：021-65161782-2601。
作者简介:李俐，女，1983年生，硕士，技师，主要从事细菌耐药性研究。

Detection and analysis of the multi-drug resistant regulator A in the clinical isolates

1. Department of Clinical Laboratory，Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine，Shanghai 200437，China; 2. Department of Clinical Laboratory，Shanghai Sixth People′s Hospital，Shanghai Jiaotong University, Shanghai 200233,China

Received:2012-04-02 Revised:2012-06-18 Online:2013-04-30 Published:2013-04-05

摘要/Abstract

摘要： 目的　调查大肠埃希菌临床菌株中多重耐药因子A (MarA)的表达情况，并分析其与第3、4代头孢菌素耐药的关系。方法　随机抽取2003至2009年间大肠埃希菌49株，采用实时定量逆转录聚合酶链反应（RT-PCR）检测外膜孔通道蛋白F（OmpF）的反义RNA-F因子（MicF）、主动外排系统AB（AcrAB）和MarA的相对表达量，纸片扩散法检测抗菌药物的敏感性。结果　49株大肠埃希菌中，7株（14.29%）AcrAB转录水平上调；13株（26.53%）膜孔蛋白MicF转录水平上调；11株（22.45%）MarA转录水平上调；3种耐药因素同时升高的有2株。49株大肠埃希菌对头孢噻肟、头孢他啶和头孢吡肟的耐药率依次为79.59%、75.51%和51.02%，其中AcrAB表达升高组对此3种抗菌药物的耐药率依次为100.00%、100.00%和57.14%，MicF表达升高组为80.00%、86.67%和53.33%，MarA表达升高组为75.00%、100.00%和58.33%，3种耐药因素同时存在时耐药率则均为100.00%。结论　MarA在大肠埃希菌临床菌种中起到一定的调控AcrAB和膜孔蛋白MicF的作用，当MarA同时调控二者高表达时可引起耐药率大幅升高。

关键词: 多重耐药因子A, 反义RNA-F因子, 外排泵, 大肠埃希菌

Abstract: Objective　To detect the expression of multi-drug resistant regulator A (MarA) in Escherichia coli，and to analyze the relationship of MarA with the resistance to 3rd and 4th generation cephalosporin. Methods　A total of 49 isolates of Escherichia coli between 2003 and 2009 were collected randomly. Real-time quantitation reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression levels of anti-putative outer membrane porin F protein (OmpF) antisense RNA-F (MicF)，efflux pump AB (AcrAB) and MarA. The antibiotic sensitivity was also analyzed by disk diffusion method. Results　Among the 49 isolates of Escherichia coli，7 of 49 isolates (14.29%) had high AcrAB transcription level，13 of 49 isolates (26.53%) had MicF transcription level，11 of 49 isolates (22.45%) had high MarA transcription level，and 2 isolates had the 3 resistant factors simultaneously with increasing level. The resistance rates to cefotaxime，ceftazidime and cefepime were 79.59%, 75.51% and 51.02%. The increasing expression level of AcrAB group showed that the resistance rates to these 3 antibiotics were 100.00%，100.00% and 57.14%，respectively. The increasing expression level of MarA group showed that the resistance rates to these 3 antibiotics were 75.00%，100.00% and 58.33%，respectively. The resistance rate increased to 100.00%，when all the 3 resistant factors existed simultaneously. Conclusions　MarA plays an important role in regulating the expressions of AcrAB and MicF. The increasing expression levels of AcrAB and MicF regulated by MarA will lead to high resistance rate to antibiotics.

Key words: Multi-drugresistantregulatorA, AntisenseRNA-F, Effluxpump, Escherichiacoli

李俐1，蒋燕群2，沈建雄1，薛志忠1. 多重耐药因子A在临床菌株中的检测与分析[J]. 检验医学, 2013, 28(4): 259-262.

LI Li 1，JIANG Yanqun 2, SHEN Jianxiong 1，XUE Zhizhong 1. Detection and analysis of the multi-drug resistant regulator A in the clinical isolates[J]. , 2013, 28(4): 259-262.

参考文献

[1]Barbosa TM，Levy SB. Differential expression of over 60 chromosomal genes in Escherichia coli by constitutive expression of MarA[J]. J Bacteriol，2000, 182(12):3467-3474.
[2]Delihas N，Forst S.MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors[J]. J Mol Biol，2001, 313(1):1-12.
[3]Aono R，Tsukagoshi N，Yamamoto M.Involvement of outer membrane protein TolC，a possible member of the mar-sox regulon，in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12[J]. J Bacteriol，1998, 180(4):938-944.
[4]Domain F，Levy SB.GyrA interacts with MarR to reduce repression of the marRAB operon in Escherichia coli[J].J Bacteriol,2011,192(4):942-948.
[5]McMurry LM,Levy SB.Evidence that regulatory protein MarA of Escherichia coli represses rob by steric hindrance[J].J Bacteriol,2010,192(15):3977-3982.
[6]Wall ME，Markowitz DA，Rosner JL,et al. Model of transcriptional activation by MarA in Escherichia coli[J]. PLoS Comput Biol，2009, 5(12): e1000614.
[7]Grkovic S，Brown MH，Skurray RA.Regulation of bacterial drug export systems[J]. Microbiol Mol Biol Rev，2002, 66(4): 671-701.
[8]Spott S，Dong F，Kisters-Woike B，et al. Dimerisation mutants of Lac repressor. Ⅱ. A single amino acid substitution，D278L，changes the specificity of dimerisation[J]. J Mol Biol，2000, 296(2): 673-684.
[9]Tkachenko AG，Pozhidaeva ON,Shumkov MS.Role of polyamines in formation of multiple antibiotic resistance of Escherichia coli under stress conditions[J]. Biochemistry (Mosc)，2006, 71(9): 1042-1049.
[10]Ramani N，Boakye K.Salicylate inhibits the translation and transcription of ompF in Escherichia coli[J]. Can J Microbiol，2001, 47(11):1053-1057.
[11]李俐,蒋燕群.大肠埃希菌临床菌株中MicF的转录情况检测及分析[J]. 中国感染与化疗杂志，2011, 11(3): 191-194.
[12]蒋燕群，李俐，钱燕斐. 大肠埃希菌和肺炎克雷伯菌外排泵转录水平与耐药的关系[J]. 检验医学，2011，26（1）：51-55.

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多重耐药因子A在临床菌株中的检测与分析

Detection and analysis of the multi-drug resistant regulator A in the clinical isolates

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