关键词: 人乳头瘤病毒, 基因型, 液基细胞学检查, 上海

Abstract:

Objective To analyze the human papillomavirus（HPV） genotypes of different cervical lesion patients in Shanghai and the results of cervical cytology tests（TCT），and to provide a reference for cervical cancer screening. Methods Totally，4 121 female HPV-infected individuals from Ruijin Hospital of Shanghai Jiao Tong University School of Medicine from July 2019 to December 2023 were enrolled. HPV genotypes were determined，and thinprep cytology test（TCT） was performed. Results Among the 4 121 female HPV-infected individuals，3 280 cases had normal TCT results，538 cases had grade Ⅰ cervical intraepithelial neoplasia （CIN），154 cases had grade Ⅱ CIN，92 cases had grade Ⅲ CIN，and 57 cases had cervical squamous cell carcinoma （SCC）. The top 3 HPV genotypes determined were HPV52 （15.83%），HPV58 （9.30%） and HPV16 （9.29%）. Among those with normal TCT results，CIN Ⅰ，CIN Ⅱ，CIN Ⅲ and SCC，HPV52 positive individuals accounted for 15.82%，15.84%，18.01%，15.11% and 12.16%，HPV58 positive individuals accounted for 8.87%，9.60%，14.22%，12.23% and 13.51%，and HPV16 positive individuals accounted for 7.00%，9.94%，30.81%，31.65% and 40.54%，respectively. The multiple HPV infection rate was 26.06%，and multiple HPV infections were found in those with normal TCT results，CIN Ⅰ，CIN Ⅱ，CIN Ⅲ and SCC，accounting for 23.48%，40.52%，25.97%，35.87% and 22.81%，respectively. Among HPV-infected individuals，high-risk HPV infection accounted for 80.40%，and high-risk HPV infection in those with normal TCT results，CIN Ⅰ，CIN Ⅱ，CIN Ⅲ and SCC accounted for 78.42%，84.51%，94.31%，94.24% and 95.95%，respectively. Conclusions The infection rate of HPV16 in CIN Ⅱ，CIN Ⅲ and SCC among female patients in Shanghai is relatively high. HPV52 is more common in those with normal TCT results and CIN Ⅰ. The HPV multiple infection rate is higher in CIN Ⅰ patients. HPV typing has certain clinical significance in the screening of cervical cancer and its precancerous lesions.

Key words: Human papillomavirus, Genotype, Thinprep cytology test, Shanghai