关键词: 微小RNA-524-5p, 微小RNA-708-5p, 喉癌, 预后

Abstract:

Objective To investigate the relationship between the levels of serum miR-524-5p and miR-708-5p and the clinicopathological characteristics and prognosis of patients with laryngeal cancer. Methods A total of 93 patients with laryngeal cancer（laryngeal cancer group） and 93 healthy subjects（healthy control group） in Nanyang Central Hospital from January 2018 to January 2021 were enrolled. The clinical data of all patients were collected，and the relative expression levels of miR-524-5p and miR-708-5p were determined. All the patients were followed up for 3 years. Pearson correlation analysis was used to analyze the correlation between miR-524-5p and miR-708-5p. The efficacy of serum miR-524-5p and miR-708-5p in the diagnosis of laryngeal cancer was evaluated by receiver operating characteristic（ROC） curve. The survival conditions of laryngeal cancer patients with different expressions of miR-524-5p and miR-708-5p were analyzed using Kaplan-Meier survival curve. Cox regression analysis was used to evaluate the influencing factors of death in patients with laryngeal cancer. Results The relative expression levels of serum miR-524-5p and miR-708-5p in laryngeal cancer group were lower than those in healthy control group（P<0.001）. The expression of serum miR-524-5p in patients with laryngeal cancer was positively correlated with that of miR-708-5p（r=0.714，P<0.05）. The areas under curves（AUC） of serum miR-524-5p and miR-708-5p in the diagnosis of laryngeal cancer were 0.801 and 0.753，respectively，and the optimal cut-off values were 0.788 and 0.813，respectively. There was statistical significance in the relative expression levels of serum miR-524-5p and miR-708-5p among laryngeal cancer patients with lymphatic metastasis，different tumor diameters，TNM stages and degrees of differentiation（P<0.05）. There was no statistical significance in the relative expression levels of serum miR-524-5p and miR-708-5p among laryngeal cancer patients of different genders，ages，tumor locations，tumor invasion depths and pathological types（P>0.05）. The optimal cut-off values of miR-524-5p and miR-708-5p were used to classify the subjects into high-expression group and low-expression group. The survival rates of both miR-524-5p high-expression group and miR-708-5p high-expression group were higher than those of their low-expression groups（P<0.05）. Lymphatic metastasis，tumor diameter>3.0 cm，low differentiation，TNM stage Ⅲ-Ⅳ，low expression of miR-524-5p and low expression of miR-708-5p were risk factors for death in patients with laryngeal cancer（P<0.05）. Conclusions The relative expression levels of serum miR-524-5p and miR-708-5p in laryngeal cancer patients are decreased，and both of them are related to clinicopathological characteristics and prognosis，which may be used as markers for prognostic assessment of laryngeal cancer.

Key words: MicroRNA-524-5p, MicroRNA-708-5p, Laryngeal cancer, Prognosis