检验医学 ›› 2015, Vol. 30 ›› Issue (8): 861-865.DOI: 10.3969/j.issn.1673-8640.2015.08.021

• 综述与讲座 • 上一篇    下一篇

呼吸道病毒实验室检测技术的研究进展

叶星晨综述, 杨海鸥, 王静审校   

  1. 上海交通大学医学院附属上海儿童医学中心检验科,上海 200127
  • 收稿日期:2014-09-09 出版日期:2015-08-30 发布日期:2015-08-28
  • 作者简介:null

    作者简介:叶星晨,女,1989年生,学士,主要从事临床检验工作。

    通讯作者:王 静,联系电话:021-38626161-5137。

  • 基金资助:
    上海交通大学医工(理)交叉研究基金资助项目(YG2012ZD03)

The research progress in respiratory virus laboratory testing technology

YE Xingchen, YANG Haiou, WANG Jing.   

  1. Department of Clinical Laboratory, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Received:2014-09-09 Online:2015-08-30 Published:2015-08-28

摘要:

呼吸道病毒是威胁人类健康的主要因素之一,因此快速准确地鉴别病原微生物具有重要的临床意义。文章介绍了目前应用于呼吸道病毒检测的一些经典或新兴实验室技术,包括病毒分离培养技术、免疫学技术、核酸扩增技术、芯片技术、下一代测序技术、质谱(MS)技术等。这些技术以其各自的特点应用于呼吸道病毒的临床诊断、疗效观察以及科学研究与探索。

关键词: 呼吸道病毒, 实验室检测方法, 研究进展

Abstract:

Respiratory virus is one of main factors that threatens human health, so it is significant to identify pathogenic microorganisms accurately and rapidly. This article introduces some traditional or new laboratory testing technologies that have been applied to respiratory virus determination nowdays, including virus isolation culture technique, immunological technique, nucleic acid sequence amplification technique, chip technology, next generation sequencing technique, mass spectrometry (MS) technique and so on. These technologies are applied into clinical diagnosis, curative effect observation, scientific research and investigation of respiratory virus by their characteristics.

Key words: Respiratory virus, Laboratory testing technology, Research progress

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