化学沉淀法测定小而密低密度脂蛋白方法的建立

doi:10.3969/j.issn.1673-8640.2014.02.014

检验医学 ›› 2014, Vol. 29 ›› Issue (2): 148-152.DOI: 10.3969/j.issn.1673-8640.2014.02.014

• 技术研究与评价·论著 • 上一篇下一篇

化学沉淀法测定小而密低密度脂蛋白方法的建立

于群¹, 王惠民¹, 季伙燕¹, 苏建友¹, 丛辉¹, 顾国浩², 沈国荣³

1. 南通大学附属医院检验医学中心, 江苏南通 226001;
2. 苏州大学附属第一医院检验科, 江苏苏州 215006; 3. 南通大学附属吴江医院检验科, 江苏吴江 215200

收稿日期:2013-04-29 出版日期:2014-02-20 发布日期:2014-02-20
通讯作者: 王惠民, 联系电话:0513-85052102
作者简介:于群, 女, 1987年生, 学士, 主要研究方向为脂蛋白分离技术。
基金资助:
江苏省“科教兴卫”医学重点学科建设资助项目(XK200723);江苏省南通市社会发展项目(S2009012)

Establishment on chemistry precipitation assay for the determination of small dense low-density lipoprotein

YU Qun¹, WANG Huimin¹, JI Huoyan¹, SU Jianyou¹, CONG Hui¹, GU Guohao², SHEN Guorong³.

1. Laboratory Medicine Center, the Affiliated Hospital of Nantong University, Jiangsu Nantong 226001, China; 2. Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Jiangsu Soochow 215006, China; 3. Department of Clinical Laboratory, Wujiang Hospital, Nantong University, Jiangsu Wujiang 215200, China

Received:2013-04-29 Online:2014-02-20 Published:2014-02-20

摘要/Abstract

摘要：

目的利用化学沉淀原理建立小而密低密度脂蛋白(sdLDL)快速、简便的检测方法。方法通过比较不同种类及不同浓度沉淀剂对血清样本中脂蛋白的分离效果, 确定最终沉淀剂, 并对化学沉淀法测定血清sdLDL-C的精密度、线性范围和回收率进行分析。结果最终选择140 U/mL肝素-90 mmol/L Mg²⁺作为沉淀剂测定血清sdLDL 胆固醇(sdLDL-C)含量。化学沉淀法测定sdLDL-C在0.14~1.44 mmol/L范围内线性关系良好, 回归方程为Y＝1.025X－0.197, r＝0.988;对高、低2组不同sdLDL-C浓度的样本批内变异系数(CV)分别为2.83%、3.19%, 批间CV分别为5.49%、6.13%;回收率为83.81%。结论 sdLDL化学沉淀法具有样本用量少、操作简便、成本低廉等优点, 更适合常规实验室检测血清sdLDL-C含量。

关键词: 小而密低密度脂蛋白, 沉淀法, 化学法

Abstract:

Objective To establish a simple and rapid method for the determination of small dense low-density lipoprotein(sdLDL) by utilizing the principle of precipitation. Methods The final and most appropriate precipitation reagents were identified through comparing the separation effect of different types and different concentrations of precipitation reagents for separating serum lipoproteins. The precision, linear range and recovery rate of chemistry precipitation assay were also analyzed. Results Finally, 140 U/mL of heparin and 90 mmol/L of Mg²⁺ as the precipitation reagents were used to determine the concentrations of serum sdLDL cholesterol(sdLDL-C). The chemistry precipitation assay was linear at 0.14-1.44 mmol/L sdLDL-C, and the regression equation was Y＝1.025X－0.197, r＝0.988. The within-run coefficients of variation(CV) of high and low sdLDL-C concentration specimens were 2.83% and 3.19%, respectively, and the between-run CV were 5.49% and 6.13%. The recovery rate was 83.81%. Conclusions The chemistry precipitation assay for the determination of sdLDL has the advantages of less serum consumption, simple operation, low cost and so on. It is more suitable for clinical laboratory to determine serum sdLDL-C.

Key words: Small dense low-density lipoprotein, Precipitation assay, Chemical method

中图分类号:

R446.1

于群, 王惠民, 季伙燕, 苏建友, 丛辉, 顾国浩, 沈国荣. 化学沉淀法测定小而密低密度脂蛋白方法的建立[J]. 检验医学, 2014, 29(2): 148-152.

YU Qun, WANG Huimin, JI Huoyan, SU Jianyou, CONG Hui, GU Guohao, SHEN Guorong.. Establishment on chemistry precipitation assay for the determination of small dense low-density lipoprotein[J]. , 2014, 29(2): 148-152.

参考文献

[1] Winkler K, Jacob S, Müller-Schewe T, et al. Ezetimibe alone and in combination lowers the concentration of small, dense low-density lipoproteins in type 2 diabetes mellitus[J]. Atherosclerosis, 2012, 220(1):189-193.
[2] Bauls C, Bellod L, Jover A, et al. Comparability of two different polyacrylamide gel electrophoresis methods for the classification of LDL pattern type[J]. Clin Chim Acta, 2012, 413(1-2):251-257.
[3] Decewicz DJ, Neatrour DM, Burke A, et al. Effects of cardiovascular lifestyle change on lipoprotein subclass profiles defined by nuclear magnetic resonance spectroscopy[J]. Lipids Health Dis, 2009, 8:26.
[4] Lu M, Gantz DL, Herscovitz H, et al. Kinetic analysis of thermal stability of human low density lipoproteins: a model for LDL fusion in atherogenesis[J]. J Lipid Res, 2012, 53(10):2175-2185.
[5] Sakurai T, Trirongjitmoah S, Nishibata Y, et al. Mea-surement of lipoprotein particle sizes using dynamic light scattering[J]. Ann Clin Biochem, 2010, 47(Pt 5):476-481.
[6] Wang H, Wang HM, Jin QH, et al. Microchip-based small, dense low-density lipoproteins assay for coronary heart disease risk assessment[J]. Electrophoresis, 2008, 29(9):1932-1941.
[7] Hirano T, Ito Y, Saegusa H, et al. A novel and simple method for quantification of small, dense LDL[J]. J Lipid Res, 2003, 44(11):2193-2201.
[8] Sharma SB, Garg S. Small dense LDL: risk factor for coronary artery disease(CAD) and its therapeutic modulation[J]. Indian J Biochem Biophys, 2012, 49(2):77-85.
[9] Ito Y, Hirano T, Yamazaki J, et al. New simple method for small, dense LDL: development of a new quantification method and clinical significance[J]. Rinsho Byori, 2004, 52(5):406-413.
[10] Hirano T, Ito Y, Koba S, et al. Clinical significance of small dense low-density lipoprotein cholesterol levels determined by the simple precipitation method[J]. Arterioscler Thromb Vasc Biol, 2004, 24(3):558-563.
[11] 张正清, 王连升, 庄兴, 等. 血清三酰甘油水平与小而密低密度脂蛋白胆固醇的关系[J]. 检验医学, 2011, 26(12):802-805.
[12] 闵芳, 吴嘉, 牛冬梅, 等. 小而密低密度脂蛋白胆固醇直接测定法与肝素镁沉淀法的比较[J]. 现代检验医学杂志, 2012, 27(4):73-75.

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化学沉淀法测定小而密低密度脂蛋白方法的建立

Establishment on chemistry precipitation assay for the determination of small dense low-density lipoprotein

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