关键词: 血铅, 丝网印刷电极法, 石墨炉原子吸收光谱法

Abstract:

Objective　To compare the applications of screen printing electrode（SPE） method and graphite furnace atomic absorption spectrophotometry（GFAAS） for blood lead determination， and to evaluate the practicability and reliability of SPE method. Methods　The SPE method was used to detect the quality control sample of the
National Center for Clinical Laboratory and whole blood samples in order to evaluate methodologically， and the results were compared with those of GFAAS. Results　　The SPE method was used to determine high， medium and low levels of blood lead， and the relative standard deviation（RSD） were 14.80%， 4.03% and 2.11%， respectively. The average recovery rate of SPE method was 103.7%. When SPE method was used to the determination of whole blood samples， there was no statistical significance compared with GFAAS（P＞0.05）. The linear regression correlation coefficient（r2） was 0.964. There was a high correlation between the 2 methods. The Bland-Altman analysis showed that the 2 methodshad consistency. With GFAAS as gold standard， the false positive rate of SPE method was 4.4%， and the false negative rate was 0.0%. Conclusions　The SPE method shows a marked performance in stability and ease of operation， and it is highly correlated with GFAAS， which is suitable for clinical bulk samples' rapid determination.

Key words: Blood lead, Screen printing electrode method, Graphite furnace atomic absorption spectrophotometry