关键词: 血铅, 芯片传感器, 微分电位溶出法, 氢化物发生原子荧光法

Abstract: Objective To evaluate the accuracy and repeatability of clinical blood lead detection by sensor chip-differential potentiometric stripping analysis and compare with hydride generation atomic fluorescence spectrometry, and to provide the reference for clinical application. Methods The 2 methods were used to analyze the blood samples and the certified reference materials (national blood lead detection external quality assessment samples in 2011). The accuracy, repeatability, recovery rate,acceptable range and coincidence rate of sensor chip-differential potentiometric stripping analysis were evaluated. Results All of the results of 6 certified reference materials were in the allowable error range(target value ?10%), and the accuracy was good. The relative standard deviations (RSD) of the low,medium and high blood lead level blood samples were 6.98%,4.09% and 2.78%, respectively.The average recovery rate was 98.1%, and the system ratio error was 1.9%. Compared the results of sensor chip-differential potentiometric stripping analysis and hydride generation atomic fluorescence spectrometry, the results had no statistical significance(P>0.05), and the correlation coefficient of linear regression analysis(r²) was 0.964. The coincidence rate of the 2 methods was 96.4%. Conclusions sensor chip-differential potentiometric stripping analysis has good accuracy, repeatability and precision, and is suitable for the clinical application of detecting blood lead.

Key words: Blood lead, Sensor chip, Differential potentiometric stripping analysis, Hydride generation atomic fluorescence spectrometry