2011至2012年徐汇区致泻性大肠埃希菌病原学监测与流行病学研究

doi:10.3969/j.issn.1673-8640.2013.08.004

检验医学 ›› 2013, Vol. 28 ›› Issue (8): 671-675.DOI: 10.3969/j.issn.1673-8640.2013.08.004

2011至2012年徐汇区致泻性大肠埃希菌病原学监测与流行病学研究

赵雪涛,高昆,张春华

上海市徐汇区疾病预防控制中心微生物检验科,上海 200237

收稿日期:2013-08-30 修回日期:2013-08-30 出版日期:2013-08-30 发布日期:2013-08-30
作者简介:赵雪涛,男,1972年生,硕士,副主任技师,主要从事微生物检验研究。

The etiological survey and epidemiology research of diagrrheagenic Escherichia coli in Xuhui district from 2011 to 2012

ZHAO Xuetao,GAO Kun,ZHANG Chunhua

Department of Microbiology Laboratory, Xuhui Center for Disease Control and Prevention, Shanghai 200237, China

Received:2013-08-30 Revised:2013-08-30 Online:2013-08-30 Published:2013-08-30

摘要/Abstract

摘要： 目的监测致泻性大肠埃希菌(DEC)在肠道传染病中的感染情况,掌握区域内各种DEC的分子流行病学特征。方法通过采集医院肠道监测点的胃肠道感染病例进行持续性的病原学监测,使用设计的10对不同毒力基因的引物探针片段进行检测后,分析各类DEC的流行病学分布特征。结果各类DEC的阳性检出率为9%[包括侵袭性大肠埃希菌(EIEC)、致病性大肠埃希菌(EPEC)、产毒性大肠埃希菌(ETEC)、肠出血性大肠埃希菌(EHEC)],如增加检测聚集性大肠埃希菌(EAEC),阳性率上升至12%; 包含EAEC和弥散性聚集大肠埃希菌(DAEC)时,阳性率为17%; 包含EAEC、耐热性肠毒素阳性时,阳性率为23%。EAEC和DAEC有明显的年龄分布特征,ETEC和DAEC的季节性特征相反,各类DEC均无性别分布差异。结论各类DEC的感染者年龄分布特点明显,月度分布呈季节性变化,加强并建立长期持续性的DEC监测是深入了解DEC分子流行病学的基础。

关键词: 致泻性大肠埃希菌, 病原学监测, 分子流行病学

Abstract: Objective To survey the infection situation of diagrrheagenic Escherichia coli(DEC) in intestinal infectious disease, and to analyze the molecular epidemiology characteristics of DEC. Methods The etiological survey was performed continuously through enrolling the cases with gastric and intestinal infectious diseases. The primer probe segements of 10 pairs of virulent genes were determined. The epidemiology distribution characteristics of DEC were analyzed. Results The positive rates of DEC were 9%[including enteroinvasive Escherichia coli(EIEC), enteropathogenic Escherichia coli(EPEC), enterotoxigenic Escherichia coli(ETEC) and enterohemorrhagic Escherichia coli(EHEC)],12%[including enteroaggregative Escherichia coli(EAEC)],17%[including EAEC and diffusively adherent Escherichia coli(DAEC)] and 23%(including EAEC and stable toxin positive). EAEC and DAEC had aging change, and ETEC and DAEC had seasonal fluctuation. DEC was independent of sex . Conclusions The DEC has aging change and seasonal fluctuation. Strengthening the etiological survey of DEC is the foundation of investigating the DEC molecular epidemiology.

Key words: Diagrrheagenic Escherichia coli, Etiological survey, Molecular epidemiology

中图分类号:

R378.2

赵雪涛,高昆,张春华. 2011至2012年徐汇区致泻性大肠埃希菌病原学监测与流行病学研究[J]. 检验医学, 2013, 28(8): 671-675.

ZHAO Xuetao,GAO Kun,ZHANG Chunhua. The etiological survey and epidemiology research of diagrrheagenic Escherichia coli in Xuhui district from 2011 to 2012[J]. , 2013, 28(8): 671-675.

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2011至2012年徐汇区致泻性大肠埃希菌病原学监测与流行病学研究

The etiological survey and epidemiology research of diagrrheagenic Escherichia coli in Xuhui district from 2011 to 2012

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