分支链DNA Alu技术定量检测败血症患者血浆循环DNA的表达

检验医学 ›› 2012, Vol. 27 ›› Issue (12): 1050-1053.

分支链DNA Alu技术定量检测败血症患者血浆循环DNA的表达

侯彦强¹,梁冬雨²,彭亮¹,娄晓丽¹,刘涛¹,陈洪卫¹

上海交通大学附属第一人民医院松江分院检验科，上海 201600

收稿日期:2012-05-24 修回日期:2012-07-30 出版日期:2012-12-30 发布日期:2012-12-04
通讯作者: 侯彦强
作者简介:侯彦强，男，1971年生，博士，副主任技师，主要从事临床感染免疫基础及检验研究。
基金资助:
上海市科委基金资助项目（12ZR1428200）

bDNA-based Alu quantitative assay of plasma cell-free DNA level in sepsis patients

Department of Clinical Laboratory，Songjiang Hospital Affiliated tofirstPeople′s Hospital，Shanghai Jiaotong University，Shanghai 201600，China

Received:2012-05-24 Revised:2012-07-30 Online:2012-12-30 Published:2012-12-04

摘要/Abstract

摘要： 目的　探讨血浆循环DNA（cf-DNA）水平定量检测在败血症诊断和败血症与全身炎症反应综合征（SIRS）鉴别诊断中的价值。方法　利用分支链DNA Alu技术定量检测24例败血症患者、43例SIRS患者和73名健康人血浆cf-DNA的表达水平；绘制受试者工作特征（ROC）曲线评估血浆cf-DNA的临床价值。结果　正常对照组血浆cf-DNA表达水平为68.56（12.56～309.78） ng/mL，SIRS组为609.67（15.98～2 596.87） ng/mL，败血症组为1 398.96（19.28～2 987.56） ng/mL，败血症组明显高于正常对照组（P＜0.001）和SIRS组（P＜0.001）。SIRS组与正常对照组、败血症组与正常对照组、败血症组与SIRS组的曲线下面积（AUC）分别为0.763（0.661～0.865）、0.955（0.884～1.025）和0.777（0.663～0.891）。结论　血浆cf-DNA具有作为新的败血症诊断标志物的临床应用价值。

关键词: 循环DNA, 分支链DNA Alu技术, 标记物, 败血症, 诊断

Abstract: Objective　To investigate the significance of plasma cell-free DNA（cf-DNA） in diagnosis of sepsis and differential diagnosis between sepsis and systemic inflammatory response syndrome（SIRS）. Methods　The plasma cf-DNA of 24 sepsis patients，43 SIRS patients and 73 healthy controls were detected by bDNA-based Alu quantitative assay. Receiver operating characteristic（ROC） curve was performed to evaluate the clinical significance of plasma cf-DNA. Results　The plasma cf-DNA significantly increased in sepsis patients compared with in SIRS patients and healthy controls [1 398.96（19.28-2 987.56）g/mL，609.67（15.98-2 596.87）ng/mL and 68.56（12.56-309.78）ng/mL，respectively，P＜0.001]. The areas under the ROC curve（AUC） of cf-DNA for the SIRS patients with healthy controls，sepsis patients with healthy controls and sepsis patients with SIRS patients were 0.763（0.661-0.865），0.955（0.884-1.025） and 0.777（0.663-0.891），respectively. Conclusions　Plasma cf-DNA has a clinical significance as a new biomarkerfor the diagnosis of sepsis.

Key words: 〖WTBZ〗Cell-free DNA, bDNA-based Alu quantitative assay, Biomarker, Sepsis, Diagnosis

侯彦强，梁冬雨，彭亮，娄晓丽，刘涛，陈洪卫. 分支链DNA Alu技术定量检测败血症患者血浆循环DNA的表达[J]. 检验医学, 2012, 27(12): 1050-1053.

HOU Yanqiang，LIANG Dongyu，PENG Liang，LOU Xiaoli，LIU Tao，CHEN Hongwei. bDNA-based Alu quantitative assay of plasma cell-free DNA level in sepsis patients[J]. , 2012, 27(12): 1050-1053.

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