关键词: p53蛋白, p300蛋白, miR-944, 非小细胞肺癌, 预后

Abstract:

Objective To investigate the roles of p53，p300 and miR-944 expressions in cancer tissues in the prognosis assessment of non-small cell lung cancer（NSCLC）patients. Methods Totally，40 NSCLC patients from Qingdao Cancer Hospital from June 2016 to June 2018 were enrolled. Cancer tissues and adjacent tissues（>5 cm away from tumor margin）were collected to determine the expressions of p53，p300 and the relative expression level of miR-944. The clinical data and laboratory determination results were collected. All the patients were followed up for 5 years and were classified into death group（10 cases）and survival group（30 cases）according to their survival status. Logistic regression analysis was used to evaluate the influencing factors of death in NSCLC patients. Receiver operating characteristic（ROC）curve was used to evaluate the efficacy of p53，p300 and miR-944 in predicting death in NSCLC patients. Kaplan-Meier survival curve was used to analyze the survival status of NSCLC patients. Results The positive rates of p53 and p300 and the relative expression level of miR-944 in cancer tissues were higher than those in adjacent tissues（P<0.05）. There was statistical significance in the positive rates of p53 and p300 and the relative expression level of miR-944 among NSCLC patients with different TNM stages，differentiation degrees and lymphatic metastasis（P<0.05）. There was statistical significance in TNM stage，smoking history，lymphatic metastasis，differentiation degree，albumin（Alb），the positive rates of p53 and p300 and the relative expression level of miR-944 between death group and survival group（P<0.05）. TNM stage Ⅲ+Ⅳ，smoking history，lymphatic metastasis，Alb≤35 g·L-1，low differentiation degree，positive p53，positive p300 and elevated miR-944 were all risk factors for death in NSCLC patients（P<0.05）. The areas under curves（AUC）of single and combined determinations of p53，p300 and miR-944 in predicting death in NSCLC patients were 0.641，0.686，0.677 and 0.878，respectively. The total survival rates of p53 negative group，p300 negative group and miR-944 low expression group were all higher than those of p53 positive group，p300 positive group and miR-944 high expression group（Log-rank χ2 values were 6.372，7.127，5.429，respectively，P<0.05）. Conclusions The p53，p300 and miR-944 are highly expressed in cancer tissues of NSCLC patients and are related to the clinical pathological characteristics and prognosis. They may serve as indicators for prognosis assessment in NSCLC patients.

Key words: P53 protein, P300 protein, MiR-944, Non-small cell lung cancer, Prognosis