血清HMGB1、IL-6水平对牙周病孕妇妊娠结局的影响

doi:10.3969/j.issn.1673-8640.2025.10.009

摘要/Abstract

摘要：

目的探究血清高迁移率族蛋白B1（HMGB1）、白细胞介素-6（IL-6）水平对牙周病孕妇妊娠结局的影响。方法选取2022年2月—2024年2月在沧州市人民医院产检且孕周<26周的患牙周病的孕妇200例（牙周病组）和牙周健康的孕妇100例（对照组）。收集所有孕妇的临床资料和高敏C反应蛋白（hs-CRP）检测结果，同时检测血清HMGB1、IL-6水平。根据妊娠结局将牙周病孕妇分为结局良好组和结局不良组。采用多因素Logistic回归分析评估牙周病孕妇不良妊娠结局的影响因素。采用受试者工作特征（ROC）曲线评价各项指标判断牙周病孕妇不良妊娠结局的效能。结果牙周病组血清HMGB1、IL-6水平显著高于对照组（P<0.001）。牙周病组早产儿、低体重儿、小于胎龄儿所占比例均高于对照组（P<0.05），新生儿体重低于对照组（P<0.001）。结局不良组并发症（妊娠糖尿病、妊娠高血压、妊娠期贫血、子痫前期）所占比例和hs-CRP、HMGB1、IL-6水平均高于结局良好组（P<0.05）。并发妊娠糖尿病、妊娠高血压、妊娠期贫血、子痫前期和hs-CRP、HMGB1、IL-6升高均是牙周病孕妇不良妊娠结局的危险因素（P<0.05）。基于多因素Logistic回归分析结果构建判断牙周病孕妇不良妊娠结局的联合预测模型。血清HMGB1、IL-6单项检测和联合预测模型判断牙周病孕妇不良妊娠结局的曲线下面积（AUC）分别为0.768、0.766、0.839。结论 HMGB1、IL-6升高可增加牙周病孕妇不良妊娠结局的风险。血清HMGB1、IL-6或可作为评估牙周病孕妇妊娠结局的生物标志物。

关键词: 高迁移率族蛋白B1, 白细胞介素-6, 牙周病, 孕妇, 妊娠结局

Abstract:

Objective To investigate the influence of serum high mobility group protein B1（HMGB1） and interleukin-6（IL-6） levels on the pregnancy outcomes of pregnant women with periodontal disease. Methods From February 2022 to February 2024，200 pregnant women with periodontal disease（periodontal disease group） and 100 pregnant women with healthy periodontal status（control group） who underwent prenatal examinations at Cangzhou People's Hospital and had a gestational age <26 weeks were enrolled. The clinical data and high-sensitivity C-reactive protein（hs-CRP）determination results were collected，and serum HMGB1 and IL-6 levels were determined. The subjects were classified into favorable and adverse outcome groups. Multivariate Logistic regression analysis was used to evaluate the influencing factors of adverse pregnancy outcomes in pregnant women with periodontal disease. Receiver operating characteristic（ROC） curve was used to evaluate the efficacy of each indicator in judging the adverse pregnancy outcomes of pregnant women with periodontal disease. Results Serum HMGB1 and IL-6 levels in periodontal disease group were higher than those in control group（P<0.001）. The proportions of preterm infants，low birth weight infants and small for gestational age infants in periodontal disease group were all higher than those in control group（P<0.05），and the new born weight was lower than that in control group（P<0.001）. The proportions of complications（gestational diabetes，gestational hypertension，gestational anemia，preeclampsia） and the levels of hs-CRP，HMGB1 and IL-6 in adverse outcome group were higher than those in favorable outcome group（P<0.05）. Gestational diabetes，gestational hypertension，gestational anemia，preeclampsia and elevated hs-CRP，HMGB1 and IL-6 were all risk factors for adverse pregnancy outcomes in pregnant women with periodontal disease（P<0.05）. Based on the results of multivariate Logistic regression analysis，a combined prediction model for judging the adverse pregnancy outcomes of pregnant women with periodontal disease was constructed. The areas under curves（AUC） of serum HMGB1，IL-6 single determinations and the combined prediction model for judging the adverse pregnancy outcomes of pregnant women with periodontal disease were 0.768，0.766 and 0.839，respectively. Conclusions Elevated levels of HMGB1 and IL-6 can increase the risk of adverse pregnancy outcomes in pregnant women with periodontal disease. Serum HMGB1 and IL-6 may serve as biomarkers for evaluating the pregnancy outcomes of pregnant women with periodontal disease.

Key words: High mobility group protein B1, Interleukin-6, Periodontal disease, Pregnant woman, Pregnancy outcome

中图分类号:

R446.1

魏粤晓, 杨丽丽, 郭丰桐. 血清HMGB1、IL-6水平对牙周病孕妇妊娠结局的影响[J]. 检验医学, 2025, 40(10): 982-986.

WEI Yuexiao, YANG Lili, GUO Fengtong. Influence of serum HMGB1 and IL-6 levels on pregnancy outcomes of pregnant women with periodontal disease[J]. Laboratory Medicine, 2025, 40(10): 982-986.

图/表 6

参考文献 19

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组别	例数	HMGB1/（ng·L^-1）	IL-6/（ng·L^-1）
牙周病组	200	794.42±170.18	48.85±9.72
对照组	100	616.75±157.72	39.04±7.05
t值		8.731	8.978
P值		<0.001	<0.001

组别	例数	HMGB1/（ng·L^-1）	IL-6/（ng·L^-1）
牙周病组	200	794.42±170.18	48.85±9.72
对照组	100	616.75±157.72	39.04±7.05
t值		8.731	8.978
P值		<0.001	<0.001

组别	例数	新生儿体重/kg	早产儿/[例（%）]	低体重儿/[例（%）]	胎儿窒息/[例（%）]	小于胎龄儿/[例（%）]
牙周病组	200	3.18±0.24	52（26.00）	57（28.50）	7（3.50）	55（27.50）
对照组	100	3.31±0.16	15（15.00）	12（12.00）	4（4.00）	13（13.00）
统计值		4.898	4.651	10.248	0.047	7.996
P值		<0.001	0.031	0.001	0.828	0.005

组别	例数	新生儿体重/kg	早产儿/[例（%）]	低体重儿/[例（%）]	胎儿窒息/[例（%）]	小于胎龄儿/[例（%）]
牙周病组	200	3.18±0.24	52（26.00）	57（28.50）	7（3.50）	55（27.50）
对照组	100	3.31±0.16	15（15.00）	12（12.00）	4（4.00）	13（13.00）
统计值		4.898	4.651	10.248	0.047	7.996
P值		<0.001	0.031	0.001	0.828	0.005

组别	例数	年龄/岁	BMI/ （kg·m^-2）	孕次/次	产次/次	分娩孕周/ 周	分娩方式
组别	例数	年龄/岁	BMI/ （kg·m^-2）	孕次/次	产次/次	分娩孕周/ 周	顺产/[例（%）]		剖宫产/[例（%）]
结局良好组	126	28.71±3.73	26.94±2.05	2±1	1±0	38.78±1.75	79（62.70）		47（37.30）
结局不良组	74	29.04±4.05	26.47±2.13	2±1	1±0	39.05±1.62	45（60.81）		29（39.19）
统计值		0.585	1.543	0.000	0.000	1.082	0.071
P值		0.559	0.124	1.000	1.000	0.28	0.791
组别	并发症						hs-CRP/ （mg·L^-1）	HMGB1/ （ng·L^-1）		IL-6/ （ng·L^-1）
组别	妊娠糖尿病/[例（%）]		妊娠高血压/[例（%）]		妊娠期贫血/[例（%）]	子痫前期/ [例（%）]	hs-CRP/ （mg·L^-1）	HMGB1/ （ng·L^-1）		IL-6/ （ng·L^-1）
结局良好组	24（19.05）		12（9.52）		13（10.32）	5（3.97）	2.34±0.41	737.05±148.34		45.74±7.39
结局不良组	25（33.78）		15（20.27）		16（21.62）	9（12.16）	2.59±0.37	892.10±157.89		54.15±8.31
统计值	5.473		4.611		4.805	4.808	4.314	6.968		7.417
P值	0.019		0.032		0.028	0.028	<0.001	<0.001		<0.001