AD患者血清核酸氧化损伤产物与抗氧化物质相关性分析

doi:10.3969/j.issn.1673-8640.2021.11.014

摘要/Abstract

摘要：

目的探讨阿尔茨海默病（AD）患者血清DNA氧化损伤产物8-氧化脱氧鸟苷（8-oxo-dGsn）和RNA氧化损伤产物8-氧化鸟苷（8-oxo-Gsn）与抗氧化物质硒、维生素（Vit）A和Vit E的相关性。方法选取55例AD患者（AD组）和64名体检健康者（正常对照组）,采用液相色谱串联质谱（LC-MS/MS）检测血清8-oxo-dGsn、8-oxo-Gsn、Vit A和Vit E水平,采用电感耦合等离子体质谱（ICP-MS）检测血清硒水平。将所有对象按年龄分为<60岁、60~69岁、70~79岁和≥80岁4个年龄段。采用Spearman相关分析评估各项指标之间的相关性。结果 AD组血清硒、Vit A水平均显著低于正常对照组（P<0.01）,血清8-oxo-dGsn水平显著高于正常对照组（P<0.01）;血清Vit E及8-oxo-dGsn水平2个组之间差异均无统计学意义（P>0.05）。正常对照组各年龄段血清硒水平均高于AD组同年龄段（P<0.01）,<60岁和60~69岁者血清Vit A水平均高于AD组同年龄段（P<0.01）,血清8-oxo-dGsn 水平则低于AD组同年龄段（P<0.05）,但70~79岁者与≥80岁者血清Vit A、8-oxo-dGsn水平差异均无统计学意义（P>0.05）。正常对照组各年龄段血清Vit E、8-oxo-Gsn水平与AD组同年龄段比较,差异均无统计学意义（P>0.05）。血清8-oxo-dGsn、8-oxo-Gsn水平与年龄呈正相关（r值分别为0.443、0.782,P<0.01）,血清硒水平与血清Vit A、Vit E水平呈正相关（r值分别为0.330、0.401,P<0.01）,血清8-oxo-dGsn水平与血清8-oxo-Gsn水平呈正相关（r=0.644,P<0.01）;其他指标两两之间均无相关性（P>0.05）。结论 AD患者DNA氧化损伤产物8-oxo-dGsn水平升高,抗氧化物质硒降低。

关键词: 8-氧化脱氧鸟苷, 8-氧化鸟苷, 硒, 维生素A, 维生素E, 阿尔茨海默病

Abstract:

Objective To investigate the relationship between serum nucleic acid oxidative damage products [DNA related substance 8-oxo-7,8-dihydro-deoxyguanosine（8-oxo-dGsn） and RNA related substance 8-oxo-7,8-dihydroguanosin（8-oxo-Gsn）] and antioxidant substance selenium,vitamin（Vit） A and Vit E in patients with Alzheimer's disease（AD）. Methods A total of 55 patients with AD and 64 apparently healthy subjects were enrolled. Liquid chromatography tandem mass spectrometry（LC-MS/MS）was used to determine the levels of 8-oxo-dGsn,8-oxo-Gsn,Vit A and Vit E in serum,and the level of selenium in serum was determined by inductively coupled plasma mass spectrometry（ICP-MS）. All the subjects were classified into 4 groups according to ages：<60 years old,60-69 years old,70-79 years old and ≥80 years old groups. Spearman correlation analysis was used to evaluate the correlation between these substances. Results The levels of selenium and Vit A in AD group were lower than those in healthy control group（P<0.01）,and the level of 8-oxo-dGsn was higher than that in healthy control group（P<0.01）. The levels of Vit E and 8-oxo-dGsn had no statistical significance between the 2 groups（P>0.05）. The selenium level in healthy control group was higher than that in AD group（P<0.01）. The level of Vit A in <60 years old and 60-69 years old groups were higher than those in AD group（P<0.01）,and the levels of 8-oxo-dGsn were lower than those in AD group（P<0.05）. There was no statistical significance in Vit A and 8-oxo-dGsn levels between 70-79 years old and ≥80 years old groups（P>0.05）. There was no statistical significance in serum Vit E and 8-oxo-Gsn levels between healthy control group and AD group（P>0.05）. 8-Oxo-dGsn and 8-oxo-Gsn levels were positively correlated with age（r=0.443 and 0.782,P<0.01）,selenium levels were positively correlated with serum Vit A and Vit E（r=0.330 and 0.401,P<0.01）,and 8-oxo-dGsn levels were positively correlated with 8-oxo-Gsn levels（r=0.644,P<0.01）. There was no correlation between the other items（P>0.05）. Conclusions Sera from patients with AD contains high levels of DNA oxidative damage product 8-oxo-dGsn and low levels of antioxidant substance selenium.

Key words: 8-Oxo-7, 8-dihydro-deoxyguanosine, 8-Oxo-7, 8-dihydroguanosin, Selenium, Vitamin A, Vitamin E, Alzheimer's disease

中图分类号:

R446.1

李文正, 禹松林, 尹逸丛, 于佳磊, 王丹晨, 邹雨桐, 邱玲. AD患者血清核酸氧化损伤产物与抗氧化物质相关性分析[J]. 检验医学, 2021, 36(11): 1164-1168.

LI Wenzheng, YU Songlin, YIN Yicong, YU Jialei, WANG Danchen, ZOU Yutong, QU Ling. Correlation between serum nucleic acid oxidative damage products and antioxidant substance in patients with Alzheimer's disease[J]. Laboratory Medicine, 2021, 36(11): 1164-1168.

图/表 2

参考文献 15

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组别	例数	硒/（μg/L）	Vit A/（mg/L）	Vit E/（mg/L）	8-oxo-dGsn/（mg/L）	8-oxo-Gsn/（mg/L）
AD组	55	72.34±3.29	0.41±0.019	7.56±0.43	0.05（0.03~0.07）	0.09（0.07~0.12）
正常对照组	64	103.61±2.97	0.57±0.018	7.81±0.28	0.03（0.02~0.04）	0.09（0.07~0.10）
统计值		0.010	0.404	5.715	4.400	0.691
P值		<0.01	<0.01	0.621	<0.01	0.490

组别	例数	硒/（μg/L）	Vit A/（mg/L）	Vit E/（mg/L）	8-oxo-dGsn/（mg/L）	8-oxo-Gsn/（mg/L）
AD组	55	72.34±3.29	0.41±0.019	7.56±0.43	0.05（0.03~0.07）	0.09（0.07~0.12）
正常对照组	64	103.61±2.97	0.57±0.018	7.81±0.28	0.03（0.02~0.04）	0.09（0.07~0.10）
统计值		0.010	0.404	5.715	4.400	0.691
P值		<0.01	<0.01	0.621	<0.01	0.490

组别	例数	硒/（μg/L）	Vit A/（mg/L）	Vit E/（mg/L）	8-oxo-dGsn/（mg/L）	8-oxo-Gsn/（mg/L）
AD组
<60岁	4	60.15±12.48^**	0.36±0.05^**	6.43±1.63	0.04（0.04~0.05）^**	0.06（0.04~0.06）
60~69岁	13	71.37±14.77^***	0.42±0.13^***	6.68±2.32	0.04（0.03~0.07）^*	0.08（0.07~0.09）
70~79岁	19	77.45±29.05^**	0.43±0.15^*	7.45±3.38	0.05（0.03~0.055）	0.09（0.075~0.12）
≥80岁	19	70.72±26.21^**	0.41±0.15^*	8.5±3.65	0.05（0.04~0.06）	0.11（0.085~0.12）
正常对照组
<60岁	14	106.09±17.27	0.55±0.13	6.89±1.46	0.02（0.02~0.03）	0.06（0.06~0.07）
60~69岁	19	103.87±17.38	0.61±0.13	8.18±2.03	0.03（0.02~0.03）	0.08（0.07~0.09）
70~79岁	17	107.29±30.74	0.56±0.18	7.21±1.67	0.04（0.02~0.05）	0.09（0.08~0.11）
≥80岁	14	96.25±19.75	0.54±0.12	8.98±3.24	0.04（0.03~0.08）	0.12（0.10~0.15）

组别	例数	硒/（μg/L）	Vit A/（mg/L）	Vit E/（mg/L）	8-oxo-dGsn/（mg/L）	8-oxo-Gsn/（mg/L）
AD组
<60岁	4	60.15±12.48^**	0.36±0.05^**	6.43±1.63	0.04（0.04~0.05）^**	0.06（0.04~0.06）
60~69岁	13	71.37±14.77^***	0.42±0.13^***	6.68±2.32	0.04（0.03~0.07）^*	0.08（0.07~0.09）
70~79岁	19	77.45±29.05^**	0.43±0.15^*	7.45±3.38	0.05（0.03~0.055）	0.09（0.075~0.12）
≥80岁	19	70.72±26.21^**	0.41±0.15^*	8.5±3.65	0.05（0.04~0.06）	0.11（0.085~0.12）
正常对照组
<60岁	14	106.09±17.27	0.55±0.13	6.89±1.46	0.02（0.02~0.03）	0.06（0.06~0.07）
60~69岁	19	103.87±17.38	0.61±0.13	8.18±2.03	0.03（0.02~0.03）	0.08（0.07~0.09）
70~79岁	17	107.29±30.74	0.56±0.18	7.21±1.67	0.04（0.02~0.05）	0.09（0.08~0.11）
≥80岁	14	96.25±19.75	0.54±0.12	8.98±3.24	0.04（0.03~0.08）	0.12（0.10~0.15）