环形RNA在肿瘤增殖与转移中的作用

doi:10.3969/j.issn.1673-8640.2020.08.022

摘要/Abstract

摘要：

环形RNA（circRNA）是一类新型的内源性非编码RNA,其在真核生物中均有广泛表达,具有物种、组织、细胞特异性,在肿瘤的发生、发展中扮演重要角色。文章主要讨论了circRNA在肿瘤的生长和转移方面的作用,并对circRNA应用于检验诊断和临床治疗的前景作出了展望。

关键词: 环状RNA, 肿瘤, 细胞周期, 上皮-间质转化

Abstract:

Circular RNA（circDNA） is a new type of endogenous non-coding RNA,which is widely expressed in eukaryotes,and it has species,tissue and cell specificities. It plays a role in the development of tumors. This review mainly discusses the role of circRNA in tumor growth and metastasis,which makes a prospect of its application in diagnosis and treatment.

Key words: Circular RNA, Tumor, Cell cycle, Epithelial-mesenchymal transformation

中图分类号:

R446.1

侯艳艳, 范基农, 刘湘帆, 倪培华. 环形RNA在肿瘤增殖与转移中的作用[J]. 检验医学, 2020, 35(8): 837-842.

HOU Yanyan, FAN Jinong, LIU Xiangfan, NI Peihua. Role of circular RNA in tumor proliferation and metastasis[J]. Laboratory Medicine, 2020, 35(8): 837-842.

图/表 2

参考文献 36

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方法	优点	局限性	参考文献
circRNA测序	有助于发现有潜力的circRNA分子	检测结果可能包括对RNase R有抗性的线性 RNA	[9]
微阵列	需要较少的生物信息学知识,是 RNA-seq可行的替代方法	只能分析有限数量的已知circRNA,,并且不能提供对应的线性对应物的信息	[10]
Northern印迹分析	可用于评估circRNA的大小、同工型、序列和丰度	操作复杂,不能用于高通量检测	[11]
实时定量PCR	实验条件简单,并且可以验证 circRNA的完整序列	存在滚环效应影响检测的精确度	[5]
数字液滴PCR	可量化circRNA拷贝数	需要专用设备和试剂	[12]
NanoString nCounter荧光条形码标记单分子检测技术	有一定的检测通量且能绝对定量	需要专用设备和试剂	[13]

方法	优点	局限性	参考文献
circRNA测序	有助于发现有潜力的circRNA分子	检测结果可能包括对RNase R有抗性的线性 RNA	[9]
微阵列	需要较少的生物信息学知识,是 RNA-seq可行的替代方法	只能分析有限数量的已知circRNA,,并且不能提供对应的线性对应物的信息	[10]
Northern印迹分析	可用于评估circRNA的大小、同工型、序列和丰度	操作复杂,不能用于高通量检测	[11]
实时定量PCR	实验条件简单,并且可以验证 circRNA的完整序列	存在滚环效应影响检测的精确度	[5]
数字液滴PCR	可量化circRNA拷贝数	需要专用设备和试剂	[12]
NanoString nCounter荧光条形码标记单分子检测技术	有一定的检测通量且能绝对定量	需要专用设备和试剂	[13]

相关蛋白/通路	circRNA	失调情况	靶标miRNA	靶标蛋白	肿瘤	参考文献
细胞周期相关蛋白/激酶
cyclin	circNR3C1	下调	miR-27a-3p	cyclin D1	膀胱癌	[14]
	has_circ_0000204	下调	miR-191	KLF6	肝癌	[15]
CKI	circ-ZKSCAN1	下调	miR-1178-3p	P21	膀胱癌	[16]
	circRNA BCRC-3	下调	miR-182-5p	P27	膀胱癌	[17]
CDK	circ-Foxo3	上调	-	P21/CDK2	乳腺癌	[18]
	circ-Ccnb1	下调	-	cyclin B1/CDK1	乳腺癌	[19]
EMT相关蛋白
EMT-TF	circPRMT5	上调	miR-30c	Snail	尿道上皮癌	[20,21]
	circNUP214	上调	miR-145	ZEB2	甲状腺乳头状癌	[22]
	circ-10720	上调	miR-490-5p	Twist1	肝细胞癌	[23]
	cirs-7	上调	miR-7	KLF4	食管鳞状细胞癌	[24]
	circNHSL1	上调	miR-1306-3p	Vimentin	胃癌	[25]
细胞黏附分子与骨架蛋白	circAMOTL1L	下调	miR-193a-5p	Pcdha8	前列腺癌	[25]
	circMTO1	上调	miR-221	N-CAD	膀胱癌	[26]
	circPTK2	下调	miR-429/miR-200b-3p	TIF1γ	非小细胞肺癌	[34]
相关信号通路
TGF-β/Smad	circRIP2	上调	miR-1305	TGF-β2	膀胱癌	[28]
Wnt/β-catenin	circMYO10	上调	miR-370-3p	RUVBL1	骨肉瘤	[29]
Notch	circNFIX	上调	miR-34a-5p	Notch1	神经胶质瘤	[30]
PI3K/Akt/mTOR	circNRIP1	上调	miR-149-5p	AKT1 / mTOR	胃癌	[31]
NF-κB	circRNA-000911	上调	miR-449a	Notch1	乳腺癌	[32]

相关蛋白/通路	circRNA	失调情况	靶标miRNA	靶标蛋白	肿瘤	参考文献
细胞周期相关蛋白/激酶
cyclin	circNR3C1	下调	miR-27a-3p	cyclin D1	膀胱癌	[14]
	has_circ_0000204	下调	miR-191	KLF6	肝癌	[15]
CKI	circ-ZKSCAN1	下调	miR-1178-3p	P21	膀胱癌	[16]
	circRNA BCRC-3	下调	miR-182-5p	P27	膀胱癌	[17]
CDK	circ-Foxo3	上调	-	P21/CDK2	乳腺癌	[18]
	circ-Ccnb1	下调	-	cyclin B1/CDK1	乳腺癌	[19]
EMT相关蛋白
EMT-TF	circPRMT5	上调	miR-30c	Snail	尿道上皮癌	[20,21]
	circNUP214	上调	miR-145	ZEB2	甲状腺乳头状癌	[22]
	circ-10720	上调	miR-490-5p	Twist1	肝细胞癌	[23]
	cirs-7	上调	miR-7	KLF4	食管鳞状细胞癌	[24]
	circNHSL1	上调	miR-1306-3p	Vimentin	胃癌	[25]
细胞黏附分子与骨架蛋白	circAMOTL1L	下调	miR-193a-5p	Pcdha8	前列腺癌	[25]
	circMTO1	上调	miR-221	N-CAD	膀胱癌	[26]
	circPTK2	下调	miR-429/miR-200b-3p	TIF1γ	非小细胞肺癌	[34]
相关信号通路
TGF-β/Smad	circRIP2	上调	miR-1305	TGF-β2	膀胱癌	[28]
Wnt/β-catenin	circMYO10	上调	miR-370-3p	RUVBL1	骨肉瘤	[29]
Notch	circNFIX	上调	miR-34a-5p	Notch1	神经胶质瘤	[30]
PI3K/Akt/mTOR	circNRIP1	上调	miR-149-5p	AKT1 / mTOR	胃癌	[31]
NF-κB	circRNA-000911	上调	miR-449a	Notch1	乳腺癌	[32]