5种中药颗粒剂与水煎剂对5种耐药菌株的体外抑菌作用比较

doi:10.3969/j.issn.1673-8640.2015.06.005

检验医学 ›› 2015, Vol. 30 ›› Issue (6): 567-570.DOI: 10.3969/j.issn.1673-8640.2015.06.005

5种中药颗粒剂与水煎剂对5种耐药菌株的体外抑菌作用比较

李蔼文, 谭俊青, 王康椿, 黄双旺, 周兵, 刘启波

广东省第二中医院检验科, 广东广州 510095

收稿日期:2015-01-15 出版日期:2015-06-30 发布日期:2015-07-03
作者简介:null
作者简介:李蔼文,女,1981年生,主管技师,主要从事医学检验工作。

Comparison on in vitro antibacterial activities for five kinds of trditional Chinese medicine granules and decoctions against five kinds of clinical drug-resistant strains

LI Aiwen, TAN Junqing, WANG Kangchun, HUANG Shuangwang, ZHOU Bing, LIU Qibo

Department of Clinical Laboratory, the Second Traditional Chinese Medicine Hospital of Guangdong, Guangdong Guangzhou 510095, China

Received:2015-01-15 Online:2015-06-30 Published:2015-07-03

摘要/Abstract

摘要： 目的

比较5种中药(黄芩、连翘、薄荷、蒲公英、苦参)颗粒剂与水煎剂对5种临床分离耐药菌株的体外抑菌效果。

方法

根据中药抗耐药菌的相关研究,选取5种有抑菌效果的中药颗粒剂与水煎剂,取血平板分离的5种临床耐药菌株[包括耐甲氧西林金黄色葡萄球菌(MRSA)、铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌及鲍曼不动杆菌],进行常规中药药敏纸片琼脂扩散法,测定抑菌圈直径,比较水煎剂和颗粒剂的抑菌效果。

结果

5种中药对5种耐药菌的抑菌强度不同,黄芩抑菌效果最好。同一种中药颗粒剂与水煎剂相比:对于MRSA,黄芩、连翘、薄荷、蒲公英、苦参2种剂型均有抑菌效果,差异无统计学意义(P>0.05);对于耐药性铜绿假单胞菌、鲍曼不动杆菌,黄芩、连翘、薄荷2种剂型均有抑菌效果,差异无统计学意义(P>0.05);对于大肠埃希菌、肺炎克雷伯杆菌,黄芩的2种剂型均有抑菌效果,差异无统计学意义(P>0.05)。

结论

同等浓度的黄芩、连翘、薄荷、蒲公英、苦参,颗粒剂和水煎剂抑菌效果无明显差异。

关键词: 中药颗粒剂, 中药水煎剂, 抑菌圈, 临床耐药菌株

Abstract: Objective

To compare in vitro antibacterial activities for 5 kinds of traditional Chinese medicine granules and decoctions (Baicalin, Forsythia, Mint, Dandelion and Sophora) against 5 kinds of clinical drug-resistant strains.

Methods

According to the related researches on traditional Chinese medicine against resistant bacteria, 5 kinds of traditional Chinese medicine granules and decoctions were selected, and 5 kinds of clinical drug-resistant strains from blood plate were collected. They included meticillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii. The disc agar diffusion method was used, and the diameter of bacteriostatic ring was measured. The bacteriostatic effects were analyzed statistically.

Results

Five traditional Chinese medicines showed different antibacterial activities, with Baicalin being the most potent. The same kind of traditional Chinese medicine granule and decoction was compared. For MRSA, Baicalin, Forsythia, Mint, Dandelion and Sophora had antibacterial effects, and there was no statistical significance (P>0.05). For Pseudomonas aeruginosa and Acinetobacter baumannii, Baicalin, Forsythia and Mint had antibacterial effects, and there was no statistical significance (P>0.05). For Escherichia coli and Klebsiella pneumoniae, Baicalin had antibacterial effects, and there was no statistical significance (P>0.05).

Conclusions

The same concentrations of Baicalin, Forsythia, Mint, Dandelion and Sophora decoctions have not obvious difference for antibacterial effect.

Key words: Traditional Chinese medicine granule, Traditional Chinese medicine decoction, Bacteriostatic ring, Clinical drug-resistant strain

中图分类号:

R446.15

李蔼文, 谭俊青, 王康椿, 黄双旺, 周兵, 刘启波. 5种中药颗粒剂与水煎剂对5种耐药菌株的体外抑菌作用比较[J]. 检验医学, 2015, 30(6): 567-570.

LI Aiwen, TAN Junqing, WANG Kangchun, HUANG Shuangwang, ZHOU Bing, LIU Qibo. Comparison on in vitro antibacterial activities for five kinds of trditional Chinese medicine granules and decoctions against five kinds of clinical drug-resistant strains[J]. Laboratory Medicine, 2015, 30(6): 567-570.

图/表 3

参考文献 13

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抗菌药物	铜绿假单胞菌	大肠埃希菌	肺炎克雷伯菌	鲍曼不动杆菌
阿米长星	R	R	R	R
氨苄西林	R	R	R	R
氨曲南	R	R	R	R
头孢他啶	R	R	R	R
头孢噻肟	R	R	R	R
头孢唑啉	R	R	R	R
庆大霉素	R	R	R	R
亚胺培南	R	S	S	S
复方磺胺甲噁唑	S	R	R	R
氨苄西林/舒巴坦	R	I	R	S

抗菌药物	铜绿假单胞菌	大肠埃希菌	肺炎克雷伯菌	鲍曼不动杆菌
阿米长星	R	R	R	R
氨苄西林	R	R	R	R
氨曲南	R	R	R	R
头孢他啶	R	R	R	R
头孢噻肟	R	R	R	R
头孢唑啉	R	R	R	R
庆大霉素	R	R	R	R
亚胺培南	R	S	S	S
复方磺胺甲噁唑	S	R	R	R
氨苄西林/舒巴坦	R	I	R	S

抗菌药物	MRSA
阿莫西林/棒酸	R
氯霉素	S
头孢唑啉	R
环丙沙星	R
庆大霉素	R
苯唑西林	R
青霉素	R
复方磺胺甲噁唑	S
万古霉素	S

抗菌药物	MRSA
阿莫西林/棒酸	R
氯霉素	S
头孢唑啉	R
环丙沙星	R
庆大霉素	R
苯唑西林	R
青霉素	R
复方磺胺甲噁唑	S
万古霉素	S

中药	剂型	MRSA	铜绿假单胞菌	大肠埃希菌	肺炎克雷伯菌	鲍曼不动杆菌
连翘	颗粒剂	11.10±0.50	7.42±0.57	-	-	6.20±0.3
	水煎剂	12.00±0.30	8.67±0.57	-	-	7.10±0.5
蒲公英	颗粒剂	7.10±0.20	-	-	-	-
	水煎剂	7.66±0.30	-	-	-	-
黄芩	颗粒剂	19.00±0.90	6.65±0.10	7.6±0.3	6.2±0.57	11.00±0.5
	水煎剂	20.36±0.50	7.00±0.20	8.1±0.2	7.1±0.10	12.10±0.5
薄荷	颗粒剂	13.70±0.14	6.50±0.59	-	-	7.60±0.2
	水煎剂	15.35±0.57	7.83±0.59	-	-	8.10±0.3
苦参	颗粒剂	6.80±0.10	-	-	-	-
	水煎剂	7.30±0.30	-	-	-	-
阳性对照		+	+	+	+	+

5种中药颗粒剂与水煎剂对5种耐药菌株的体外抑菌作用比较

Comparison on in vitro antibacterial activities for five kinds of trditional Chinese medicine granules and decoctions against five kinds of clinical drug-resistant strains

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