miRNA-195在膀胱癌细胞中功能的研究

doi:10.3969/j.issn.1673-8640.2014.05.024

检验医学 ›› 2014, Vol. 29 ›› Issue (5): 540-544.DOI: 10.3969/j.issn.1673-8640.2014.05.024

miRNA-195在膀胱癌细胞中功能的研究

陈婧¹, 孙奋勇¹, 马纪²

1. 同济大学附属上海市第十人民医院检验科, 上海 200072;
2. 同济大学附属第十人民医院中心实验室, 上海 200072

收稿日期:2013-12-30 出版日期:2014-05-30 发布日期:2014-05-27
通讯作者: 孙奋勇, 电话:021-66306909。
作者简介:陈婧, 女, 1989年生, 学士, 主要从事肿瘤及其分化的研究。

Research on the function of miRNA-195 on bladder cancer cell

CHEN Jing¹, SUN Fenyong², MA Ji³.

1. Department of Clinical Laboratory, Shanghai Tenth People′s Hospital, Tongji University, Shanghai 200072, China;
2. Department of Central Laboratory, Shanghai Tenth People′s Hospital, Tongji University, Shanghai 200072, China

Received:2013-12-30 Online:2014-05-30 Published:2014-05-27

摘要/Abstract

摘要： 目的研究微小RNA(miRNA)-195对人膀胱癌细胞5637增殖、迁移及侵袭能力的影响。方法利用阳离子脂质体方法将miRNA-195转染至人膀胱癌细胞5637(miRNA-195组), 同时以只转染病毒载体的5637细胞作为对照组。采用实时荧光定量聚合酶链反应(PCR)检测转染细胞中miRNA-195的表达量。采用噻唑蓝(MTT)法、细胞计数法和集落形成实验测定miRNA-195对5637细胞体外增殖的影响。采用划痕试验、Transwell实验、Matrigel 肿瘤细胞侵袭实验检测miRNA-195对 5637细胞迁移、侵袭能力的影响。结果实时荧光定量PCR检测结果表明转染后的5637细胞高表达miRNA-195。MTT法、细胞计数法及集落形成实验证明miRNA-195可以抑制5637细胞在体外的增殖, miRNA-195组和对照组比较差异有统计学意义(P<0.05)。划痕实验、Transwell实验以及Matrigel肿瘤细胞侵袭实验证明miRNA-195可以抑制5637细胞的迁移和侵袭能力, miRNA-195组和对照组比较差异有统计学意义(P<0.05)。结论 miRNA-195能够抑制膀胱癌细胞5637在体外的增殖、迁移以及侵袭能力。

关键词: 微小RNA-195, 人膀胱癌细胞, 细胞增殖, 细胞迁移

Abstract: Objective To investigate the influence of microRNA (miRNA)-195 on the proliferation, migration and invasion of human bladder cancer cell 5637. Methods Human bladder cancer cell 5637 was transfected with miRNA-195 by cationic liposomes as miRNA-195 group and transfected with the vector as control group. The expression of miRNA-195 was determined by real-time fluorescence quantitation polymerase chain reaction (PCR). Methyl thiazolyl tetrazolium(MTT) assay, cell counting assay and colony formation assay were used to detect the cell proliferation . Cell migration and invasion were determined by wound healling assay, Transwell assay and Matrigel tumour invasion assay. Results Real-time fluorescence quantitation PCR showed that there was high expression of miRNA-195 after transfection of cell 5637. MTT assay, cell counting assay and colony formation assay demonstrated that miRNA-195 can suppress cell 5637 proliferation in vitro, and there were statistical significance between miRNA-195 and control groups(P<0.05).Wound healing assay, Transwell assay and tumour invasion assay showed that miRNA-195 can suppress cell migration and invasion with statistical significance between the 2 groups(P<0.05). Conclusions MiRNA-195 has inhibitory ability on the proliferation, migration and invasion of bladder cancer cell 5637 cell.

Key words: Micro RNA-195, Human bladder cancer cell, Cell proliferation, Cell migration

中图分类号:

陈婧, 孙奋勇, 马纪. miRNA-195在膀胱癌细胞中功能的研究[J]. 检验医学, 2014, 29(5): 540-544.

CHEN Jing, SUN Fenyong, MA Ji.. Research on the function of miRNA-195 on bladder cancer cell[J]. , 2014, 29(5): 540-544.

参考文献

[1] Van Roekel EH, Cheng KK, James ND, et al. Smoking is associated with lower age, higher grade, higher stage, and larger size of malignant bladder tumors at diagnosis[J]. Int J Cancer, 2013, 133(2):446-454.
[2] Kiriluk KJ, Prasad SM, Patel AR, et al. Bladder cancer risk from occupational and environmental exposures[J]. Urol Oncol, 2012, 30(2):199-211.
[3] Catto JW, Alcaraz A, Bjartell AS, et al. MicroRNA in prostate, bladder, and kidney cancer: a systematic review[J]. Eur Urol, 2011, 59(5):671-681.
[4] Bo J, Yang G, Huo K, et al. microRNA-203 suppresses bladder cancer development by repressing bcl-w expression[J]. FEBS J, 2011, 278(5):786-792.
[5] Majid S, Dar AA, Saini S, et al. MicroRNA-23b functions as a tumor suppressor by regulating Zeb1 in bladder cancer[J]. PLoS One, 2013, 8(7):e67686.
[6] Xu X, Chen H, Lin Y, et al. MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met[J]. Mol Cells, 2013, 36(1):62-68.
[7] Hui W, Yuntao L, Lun L, et al.MicroRNA-195 inhibits the proliferation of human glioma cells by directly targeting cyclin D1 and cyclin E1[J]. PLoS One, 2013, 8(1):e54932.
[8] Deng H, Guo Y, Song H, et al.MicroRNA-195 and microRNA-378 mediate tumor growth suppression by epigenetical regulation in gastric cancer[J]. Gene, 2013, 518(2):351-359.
[9] Zaravinos A, Radojicic J, Lambrou GI, et al.Expression of miRNAs involved in angiogenesis, tumor cell proliferation, tumor suppressor inhibition, epithelial-mesenchymal transition and activation of metastasis in bladder cancer[J]. J Urol, 2012, 188(2):615-623.
[10] Guancial EA, Bellmunt J, Yeh S, et al. The evolving understanding of microRNA in bladder cancer[J]. Urol Oncol, 2013, 32(1):e31-e40.
[11] Wang R, Zhao N, Li S, et al.MicroRNA-195 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting the expression of VEGF, VAV2, and CDC42[J]. Hepatology, 2013, 58(2):642-653.
[12] Mao JH, Zhou RP, Peng AF, et al. microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN[J]. Oncol Lett, 2012, 4(5):1125-1129.
[13] 蒋海锋, 薄隽杰. MicroRNA与膀胱癌的研究进展[J]. 中国癌症杂志, 2011, 21(4): 308-312.
[14] Ichimi T, Enokida H, Okuno Y, et al. Identification of novel microRNA targets based on microRNA signatures in bladder cancer[J]. Int J Cancer, 2009, 125(2): 345-352.
[15] Huang L, Luo J, Cai Q, et al. MicroRNA-125b suppresses the development of bladder cancer by targeting E2F3[J]. Int J Cancer, 2011, 28(8): 1758-1769.
[16] Lu Q, Lu C, Zhou GP, et al. MicroRNA-221 silencing predisposed human bladder cancer cells to undergo apoptosis induced by TRAIL[J]. Urol Oncol, 2010, 28(6): 635-641.

miRNA-195在膀胱癌细胞中功能的研究

Research on the function of miRNA-195 on bladder cancer cell

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