SYSMEX XN-1000全自动血液分析仪计数有核红细胞的准确性评价

doi:10.3969/j.issn.1673-8640.2014.03.015

检验医学 ›› 2014, Vol. 29 ›› Issue (3): 262-265.DOI: 10.3969/j.issn.1673-8640.2014.03.015

SYSMEX XN-1000全自动血液分析仪计数有核红细胞的准确性评价

王也飞，周怡，丁磊，夏文权，胡翊群

上海交通大学医学院附属瑞金医院检验系，上海 200025

收稿日期:2013-08-12 出版日期:2014-03-30 发布日期:2014-04-19
作者简介:王也飞,女,1974年生,硕士,讲师,主要从事临床血液学检验教学和研究。

The accuracy evaluation of nucleated red blood cell count determined by SYSMEX XN-1000 automatic hematology analyzer

WANG Yefei,ZHOU Yi,DING Lei,XIA Wenquan,HU Yiqun.

Faculty of Medical Laboratory Science,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China

Received:2013-08-12 Online:2014-03-30 Published:2014-04-19

摘要/Abstract

摘要：

目的　探讨SYSMEX XN-1000全自动血液分析仪(简称XN-1000)计数有核红细胞(NRBC)的准确性。方法　应用XN-1000对66例外周血标本进行NRBC检测,同时用流式细胞仪(FCM)和显微镜(镜检法)对同一份外周血的NRBC百分率进行计数。以镜检法为金标准,比较不同方法的计数结果。结果　XN-1000与镜检法检测NRBC有较好的相关性(r=0.927),但低于FCM与镜检法NRBC的相关性(r=0.956),XN-1000与FCM检测NRBC的相关性较高(r=0.994)。XN-1000、FCM计数结果与镜检法的结果间差异均无统计学意义(P>0.05)。XN-1000检测NRBC%较FCM法有更高的诊断特异性(95.7%)。结论　XN-1000能快速、准确、有效地自动计数外周血NRBC。

关键词: Sysmex XN-1000全自动血液分析仪, 有核红细胞, 准确性

Abstract:

Objective　To investigate the accuracy of nucleated red blood cell(NRBC) count determined by SYSMEX XN-1000 automatic hematology analyzer(XN-1000). Methods　A total of 66 peripheral blood samples were collected and determined for NRBC by XN-1000,and the results of NRBC% were compared with those by flow cytometry (FCM) and manual microscopy for the same sample. The manual microscopy was as golden standard. Results　There was a good correlation for NRBC between XN-1000 and microscopy(r=0.927),but the correlation was lower than that between FCM and manual microscopy (r=0.956).The correlation between XN-1000 and FCM was the best (r=0.994). The results of XN-1000 and FCM with manual microscopy had no statistical significance(P>0.05). XN-1000 for NRBC% had a better specificity (95.7%) than FCM. Conclusions　XN-1000 automatic hematology analyzer has good performance for detecting NRBC in peripheral blood rapidly,accurately and effectively.

Key words: Sysmex XN-1000 automatic hematology analyzer, Nucleated red blood cell, Accuracy

中图分类号:

R446.11

王也飞，周怡，丁磊，夏文权，胡翊群. SYSMEX XN-1000全自动血液分析仪计数有核红细胞的准确性评价[J]. 检验医学, 2014, 29(3): 262-265.

WANG Yefei, ZHOU Yi, DING Lei, XIA Wenquan, HU Yiqun.. The accuracy evaluation of nucleated red blood cell count determined by SYSMEX XN-1000 automatic hematology analyzer[J]. , 2014, 29(3): 262-265.

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SYSMEX XN-1000全自动血液分析仪计数有核红细胞的准确性评价

The accuracy evaluation of nucleated red blood cell count determined by SYSMEX XN-1000 automatic hematology analyzer

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