反复喘息婴幼儿外周血Tc9细胞检测及其临床意义

doi:10.3969/j.issn.1673-8640.2018.04.002

摘要/Abstract

摘要：

目的检测反复喘息婴幼儿外周血Tc9细胞比例,探讨其在疾病过程中的作用。方法收集34例反复喘息婴幼儿（喘息组）和34名健康婴幼儿（对照组）外周血,采用流式细胞仪检测Tc9细胞比例,逆转录聚合酶链反应（PCR）检测外周血单个核细胞（PBMC）白细胞介素（IL）-9 mRNA及干扰素调节因子4（IRF4）mRNA转录水平,LUMINEX 200液相芯片分析系统检测血清中细胞因子IL-9、IL-4、IL-33、IL-17A和转化生长因子（TGF）-β1水平。结果喘息组外周血Tc9细胞比例[2.01（0.67,4.52）%]较对照组[0.76（0.21,1.46）%]明显升高（P<0.05）,PBMC IL-9 mRNA和IRF4 mRNA水平亦高于对照组（P<0.05）,且与Tc9细胞比例呈正相关（r=0.501,P=0.002;r=0.504,P=0.002）。喘息组外周血细胞因子IL-9、TGF-β1、IL-4和IL-33水平均高于对照组（P<0.05）,但IL-17A水平与对照组比较,差异无统计学意义（P>0.05）。喘息组外周血Tc9细胞比例与血清IgE、IL-9呈正相关（r=0.354,P=0.039;r=0.459,P=0.006）,与IL-33、IL-4和TGF-β1无相关性（r=0.241,P=0.169;r=0.252,P=0.153;r=0.236,P=0.178）。结论外周血Tc9细胞在反复喘息婴幼儿发病过程中可能发挥重要作用。

关键词: Tc9细胞, 白细胞介素9, 白细胞介素33, 反复喘息, 婴幼儿

Abstract:

Objective To investigate the role of Tc9 cells in the pathogenesis of recurrent wheezing,through determining the proportion of Tc9 cells in peripheral blood. Methods A total of 34 infants with recurrent wheezing were enrolled as wheezing group,and 34 age-matched healthy infants were enrolled as control group. The proportion of Tc9 cells was determined by flow cytometry. The levels of peripheral blood mononuclear cell（PBMC） interleukin（IL）-9 mRNA and interferon regulatory factor 4（IRF4） mRNA were determined by reverse transcription polymerase chain reaction（PCR）. The cytokines（IL-9,IL-17A,IL-4 and IL-33） and transforming growth factor（TGF）-beta 1 were determined by LUMINEX 200 liquid phase chip analysis system. Results The proportion of Tc9 cells in wheezing group [2.01（0.67,4.52）%] was higher than that in control group [0.76（0.21,1.46）%]（P<0.05）. PBMC IL-9 mRNA and IRF4 mRNA levels in wheezing group were higher than those in control group（P<0.05）,which showed positive correlations with the proportion of Tc9 cells（r=0.501,P=0.002;r=0.504,P=0.002）. Compared to control group,there were higher levels of IL-9,IL-4,IL-33 and TGF-beta 1 in wheezing group（P<0.05）. The levels of IL-17A had no statistical significance between the 2 groups（P>0.05）. The proportion of Tc9 cells showed positive correlations with the levels of serum IgE（r=0.354,P=0.039） and IL-9（r=0.459,P=0.006） and no correlation with the levels of IL-33,IL-4 and TGF-beta 1（r=0.241,P=0.169;r=0.252,P=0.153;r=0.236,P=0.178）. Conclusions Peripheral blood Tc9 cell may play a role in the pathogenesis of recurrent wheezing.

Key words: Tc9 cell, Interleukin 9, Interleukin 33, Recurrent wheezing, Infant

中图分类号:

R446.1

于建秀, 钱雷, 徐东晖, 刘光亮. 反复喘息婴幼儿外周血Tc9细胞检测及其临床意义[J]. 检验医学, 2018, 33(4): 279-284.

YU Jianxiu, QIAN Lei, XU Donghui, LIU Guangliang. Determination of Tc9 cells in peripheral blood of infants with recurrent wheezing[J]. Laboratory Medicine, 2018, 33(4): 279-284.

图/表 7

参考文献 21

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组别	例数	年龄（岁）	性别（男/女）	总 IgE （ng/mL）		淋巴细胞（×10⁹/L）		嗜酸性粒细胞（×10⁹/L）
喘息组	34	1.5±0.6	24/10	275.4±97.2^*		5.27±1.22		0.30±0.13^*
对照组	34	1.6±0.6	23/11	124.6±35.2		5.18±1.42		0.18±0.07
组别	过敏原[例（%）]
组别	鱼、虾、蟹	牛奶	花生、大豆	鸡蛋	尘螨		猫毛、狗毛	合计
喘息组	1（2.94）	3（8.82）	1（2.94）	2（5.88）	3（8.82）		1（2.94）	11（32.4 ）
对照组	0（0.00）	0（0.00）	0（0.00）	0（0.00）	0（0.00）		0（0.00）	0（0.00）

组别	例数	年龄（岁）	性别（男/女）	总 IgE （ng/mL）		淋巴细胞（×10⁹/L）		嗜酸性粒细胞（×10⁹/L）
喘息组	34	1.5±0.6	24/10	275.4±97.2^*		5.27±1.22		0.30±0.13^*
对照组	34	1.6±0.6	23/11	124.6±35.2		5.18±1.42		0.18±0.07
组别	过敏原[例（%）]
组别	鱼、虾、蟹	牛奶	花生、大豆	鸡蛋	尘螨		猫毛、狗毛	合计
喘息组	1（2.94）	3（8.82）	1（2.94）	2（5.88）	3（8.82）		1（2.94）	11（32.4 ）
对照组	0（0.00）	0（0.00）	0（0.00）	0（0.00）	0（0.00）		0（0.00）	0（0.00）

组别	例数	Tc9细胞比例（%）	IL-9 mRNA	IRF4 mRNA
喘息组	34	2.01（0.67,4.52）^*	1.81（0.47,2.52）^*	3.67（1.84,5.97）^*
对照组	34	0.76（0.21,1.46）	1.07（0.93,1.36）	1.21（1.07,1.51）

组别	例数	Tc9细胞比例（%）	IL-9 mRNA	IRF4 mRNA
喘息组	34	2.01（0.67,4.52）^*	1.81（0.47,2.52）^*	3.67（1.84,5.97）^*
对照组	34	0.76（0.21,1.46）	1.07（0.93,1.36）	1.21（1.07,1.51）

组别	例数	IL-9（pg/mL）	IL-17A（pg/mL）	IL-33（pg/mL）	TGF-β1（pg/mL）	IL-4（pg/mL）
喘息组	34	7.53（6.01,14.92）^*	42.1（24.5,51.2）	82.4（79.2,103.8）^*	65.3（60.0,69.9）^*	650.6（565.3,725.6）^*
对照组	34	6.23（5.91,7.00）	38.7（23.3,48.5）	60.1（52.3,77.6）	32.5（28.2,45.4）	512.3（439.2,587.6）