Effects of miR-24-3p targeting CHD5 on migration，invasion and radiosensitivity of thyroid cancer cells

doi:10.3969/j.issn.1673-8640.2023.07.005

Abstract

Abstract:

Objective To investigate the effects of microRNA（miR）-24-3p regulating the expression of chromodomain helicase DNA-binding protein 5（CHD5） on the migration，invasion and radiosensitivity of thyroid cancer cells. Methods A total of 125 patients with thyroid cancer were enrolled in Xiaoshan First People's Hospital from September 2018 to September 2020. Cancer tissues，paracancer tissues （4 cm from tumor margin） and clinicopathologic data were collected. Human normal thyroid cell line Nthy-ori 3-1 and human thyroid cancer cell lines，KTC1，SW579 and TPC-1，were selected. The expressions of miR-24-3p and CHD5 proteins in cancer tissue，paracancer tissue and 4 kinds of cells were determined. SW579 cells were transfected with miR-24-3p inhibitor anti-miR-24-3p（anti-miR-24-3p group），negative control anti-miR-NC （anti-miR-NC group），miR-24-3p mimic miR-24-3p mimics （miR-24-3p mimics group），negative control miR-NC （miR-NC group），co-transfected anti-miR-24-3p and si-NC （anti-miR-24-3p+si-NC group），co-transfected anti-miR-24-3p and si-CHD5 （anti-miR-24-3p+si-CHD5 group）. The migration ability，proliferation ability and radiosensitivity of each group were determined. Dual luciferase reporter assay was used to verify the targeting relationship between miR-24-3p and CHD5. Results The relative expression level of miR-24-3p in cancer tissue and thyroid cancer cell lines（KTC1，SW579 and TPC-1） were higher than those in paracancer tissue and normal thyroid cell line Nthy-ori 3-1（P<0.05）. The relative expression level of CHD5 protein was lower than those in paracancer tissue and normal thyroid cell line Nthy-ori 3-1（P<0.05）. SW579 cells had the highest relative expression of miR-24-3p and the lowest relative expression of CHD5 protein. The expressions of miR-24-3p and CHD5 proteins had statistical significance in thyroid cancer patients with or without lymphatic metastasis and different clinical stages（P<0.001）. The inhibition of miR-24-3p expression could inhibit the migration and invasion abilities of SW579 cells，and the radiosensitivity of SW579 cells was enhanced（P<0.05）. The targeting of miR-24-3p regulated CHD5 expression negatively. Silencing CHD5 could reverse the effects of miR-24-3p expression on migration，invasion and radiosensitivity of SW579 cells（P<0.05）. Conclusions The inhibition of miR-24-3p expression can inhibit the migration and invasion abilities of SW579 cells and enhance their radiosensitivity by up-regulating CHD5.

Key words: MicroRNA-24-3p, Chromodomain helicase DNA-binding protein 5, Radiosensitivity, Cell migration, Cell invasion, Thyroid cancer

CLC Number:

R393

PAN Xin, LÜ Ying, LI Yang, LI Rongguo. Effects of miR-24-3p targeting CHD5 on migration，invasion and radiosensitivity of thyroid cancer cells[J]. Laboratory Medicine, 2023, 38(7): 640-646.

Figures/Tables 14

References 20

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临床病例特征	例数	miR-24-3p	CHD5蛋白
性别
男性	40	2.27±0.31	0.18±0.05
女性	85	2.24±0.28	0.17±0.01
t值		0.540	0.915
P值		0.590	0.362
年龄
≤60岁	65	2.29±0.38	0.17±0.01
>60岁	60	2.21±0.19	0.17±0.03
t值		1.470	0.000
P值		0.144	1.000
淋巴转移
有	52	2.35±0.32	0.15±0.03
无	73	2.11±0.25	0.20±0.01
t值		4.704	13.261
P值		<0.001	<0.001
临床分期
Ⅰ期	78	2.05±0.27	0.14±0.04
Ⅱ~Ⅲ期	47	2.37±0.30	0.19±0.01
t值		6.154	10.532
P值		<0.001	<0.001

临床病例特征	例数	miR-24-3p	CHD5蛋白
性别
男性	40	2.27±0.31	0.18±0.05
女性	85	2.24±0.28	0.17±0.01
t值		0.540	0.915
P值		0.590	0.362
年龄
≤60岁	65	2.29±0.38	0.17±0.01
>60岁	60	2.21±0.19	0.17±0.03
t值		1.470	0.000
P值		0.144	1.000
淋巴转移
有	52	2.35±0.32	0.15±0.03
无	73	2.11±0.25	0.20±0.01
t值		4.704	13.261
P值		<0.001	<0.001
临床分期
Ⅰ期	78	2.05±0.27	0.14±0.04
Ⅱ~Ⅲ期	47	2.37±0.30	0.19±0.01
t值		6.154	10.532
P值		<0.001	<0.001

组别	miR-24-3p	迁移细胞数/（个/高倍镜视野）	侵袭细胞数/（个/高倍镜视野）
anti-miR-NC组	1.04±0.12	89.98±6.32	67.25±4.12
anti-miR-24-3p组	0.32±0.04	36.25±2.83	28.52±2.33
t值	13.943	19.006	20.043
P值	<0.001	<0.001	<0.001

组别	miR-24-3p	迁移细胞数/（个/高倍镜视野）	侵袭细胞数/（个/高倍镜视野）
anti-miR-NC组	1.04±0.12	89.98±6.32	67.25±4.12
anti-miR-24-3p组	0.32±0.04	36.25±2.83	28.52±2.33
t值	13.943	19.006	20.043
P值	<0.001	<0.001	<0.001

组别	存活率/%
组别	0 Gy	2 Gy	4 Gy	6 Gy	8 Gy
anti-miR-NC组	99.81±0.22	87.53±3.31	72.91±2.10	36.80±1.42	7.34±0.82
anti-miR-24-3p组	99.92±0.11	68.24±2.93	8.62±0.33	1.24±0.22	0.40±0.13
t值	1.095	10.689	74.080	60.618	20.475
P值	0.299	<0.001	<0.001	<0.001	<0.001