Analysis of common patterns of HIV antibody banding by western blotting and its role in follow-up period

doi:10.3969/j.issn.1673-8640.2020.02.001

Abstract

Abstract:

Objective To study the common patterns of human immunodeficiency virus（HIV） antibody by western blotting and its role in follow-up period. Methods A total of 460 HIV infection patients determined by western blotting and 16 HIV antibody indeterminant individuals were enrolled. The clinical data were analyzed retrospectively. Results Among the 460 HIV infection cases,the determination rates of HIV gp160,gp120 and gp41 in env antigen bands were 100.00%,99.35% and 96.30%,respectively. In pol antigen bands,the determination rates of HIV p66,p51 and p31 were 92.61%,86.96% and 91.96%,respectively. However,antibody reactivities to gag antigen varied,being 56.30%,13.26%,100.00% and 87.17% for p55,p39,p24 and p17,respectively. Totally,34 banding patterns were determined,and the most commonly observed patterns included gp160/gp120/p66/p55/p51/gp41/p31/p24/p17（absence of reactivity to p39,accounting for 41.74%）,gp160/gp120/p66/p51/gp41/p31/p24/p17（absence of reactivity to p55 and p39,accounting for 23.70%） and all of the bands（accounting for 11.30%）. Totally,16 HIV antibody indeterminant cases were followed up,and 4 HIV antibody indeterminant cases were converted to HIV antibody positive. The main source department of HIV infection was internal medicine department（66.31%） among the involved 28 clinical departments. Conclusions There are certain differences in the determination rate of western blotting bands among HIV infection patients. There are many departments with the first diagnosis of HIV infection. The medical institutions should pay attention to HIV determination and avoid the misdiagnosis and missed diagnosis of acquired immune deficiency syndrome（AIDS）.

Key words: Human immunodeficiency virus, Western blotting, Banding pattern, Follow-up, Clinical department

CLC Number:

R446.1

WANG Wanhai, MENG Zhen, YANG Ruonan, GAO Nan, WANG Qingfang, ZHANG Lei, WANG Meifen, MING Liang. Analysis of common patterns of HIV antibody banding by western blotting and its role in follow-up period[J]. Laboratory Medicine, 2020, 35(2): 95-99.

Figures/Tables 3

References 14

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gp160	gp120	p66	p55	p51	gp41	p39	p31	p24	p17	例数	百分比/%
+	+	+	+	+	+		+	+	+	192	41.74
+	+	+		+	+		+	+	+	109	23.70
+	+	+	+	+	+	+	+	+	+	52	11.30
+	+	+		+	+		+	+		26	5.65
+	+	+			+		+	+	+	11	2.39
+	+				+			+	+	7	1.52
+	+	+			+		+	+		6	1.30
+	+				+			+		5	1.09
+	+	+	+	+	+		+	+		4	0.87
+	+	+	+		+		+	+	+	4	0.87
+	+	+	+		+	+	+	+	+	4	0.87
+	+							+		4	0.87
+	+				+		+	+	+	4	0.87
+	+	+		+	+			+		3	0.65
+	+							+	+	3	0.65
+	+				+		+	+		3	0.65
+	+	+	+	+	+			+	+	2	0.43
+	+	+			+			+	+	2	0.43
+	+	+			+			+		2	0.43
+	+			+				+		2	0.43
+	+			+	+		+	+	+	2	0.43
+		+						+	+	1	0.22
+								+	+	1	0.22
+					+			+	+	1	0.22
+	+	+	+	+	+	+		+	+	1	0.22
+	+	+	+	+	+	+		+		1	0.22
+	+	+	+		+	+	+	+	+	1	0.22
+	+	+		+				+		1	0.22
+	+	+	+		+		+	+		1	0.22
+	+	+		+	+			+	+	1	0.22
+	+	+					+	+		1	0.22
+	+	+			+	+	+	+	+	1	0.22
+	+		+		+		+	+	+	1	0.22
+	+				+	+	+	+	+	1	0.22

gp160	gp120	p66	p55	p51	gp41	p39	p31	p24	p17	例数	百分比/%
+	+	+	+	+	+		+	+	+	192	41.74
+	+	+		+	+		+	+	+	109	23.70
+	+	+	+	+	+	+	+	+	+	52	11.30
+	+	+		+	+		+	+		26	5.65
+	+	+			+		+	+	+	11	2.39
+	+				+			+	+	7	1.52
+	+	+			+		+	+		6	1.30
+	+				+			+		5	1.09
+	+	+	+	+	+		+	+		4	0.87
+	+	+	+		+		+	+	+	4	0.87
+	+	+	+		+	+	+	+	+	4	0.87
+	+							+		4	0.87
+	+				+		+	+	+	4	0.87
+	+	+		+	+			+		3	0.65
+	+							+	+	3	0.65
+	+				+		+	+		3	0.65
+	+	+	+	+	+			+	+	2	0.43
+	+	+			+			+	+	2	0.43
+	+	+			+			+		2	0.43
+	+			+				+		2	0.43
+	+			+	+		+	+	+	2	0.43
+		+						+	+	1	0.22
+								+	+	1	0.22
+					+			+	+	1	0.22
+	+	+	+	+	+	+		+	+	1	0.22
+	+	+	+	+	+	+		+		1	0.22
+	+	+	+		+	+	+	+	+	1	0.22
+	+	+		+				+		1	0.22
+	+	+	+		+		+	+		1	0.22
+	+	+		+	+			+	+	1	0.22
+	+	+					+	+		1	0.22
+	+	+			+	+	+	+	+	1	0.22
+	+		+		+		+	+	+	1	0.22
+	+				+	+	+	+	+	1	0.22

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