Abstract:

Objective To understand the infection and gene subtype distribution of human papillomavirus（HPV） women in Fuzhou,and to provide a reference for the prevention,diagnosis and treatment of cervical cancer in this area. Methods From February 2014 to December 2017,18 542 women from Fujian Provincial Hospital were enrolled. Universal primer multiplex polymerase chain reaction（PCR） and flow fluorescence hybridization were used to determine 27 HPV gene subtypes in female cervical exfoliated cell samples. Results There were 2 902 cases of HPV positive in 18 542 physical examination women,the positive rate was 15.7%,and 27 gene subtypes were determined. The top 4 high-risk gene subtypes were 52,58,53 and 16,and the top 4 low-risk gene subtypes were 61,81,44 and 55. Totally,2 267 cases（78.1%） were infected with a single type,and 493 cases（17.0%） were double-infected. HPV positive rates of different age groups had statistical significance（P<0.05）. Conclusions The high-risk gene subtypes of HPV infection in physical examination women in Fuzhou are 52,58,53 and 16. The single infection and double infection are the main types of infection. HPV screening over 50-year-old women should be strengthened,which would be of significance for the early detection,prevention and treatment of cervical cancer.

Key words: Human papillomavirus, Gene subtype, Cervical cancer