Influence of neonatal blood sample hemolysis on the determination of serum neuron-specific enolase and establishment of correction equation

doi:10.3969/j.issn.1673-8640.2019.01.009

Abstract

Abstract:

Objective To evaluate the influence of neonatal blood sample hemolysis on the determination of serum neuron-specific enolase（NSE）,and to establish correction equation. Methods Chemiluminescence was used to determine the change of serum NSE level before and after adding self-frozen thawed red blood cells in 72 neonates borned less than 24 h. The changes of hemoglobin（Hb） before and after adding were determined by cyanmethemoglobin（HiCN）. The correction equation was established. The feasibility of correction equation was evaluated by comparing the NSE results with the NSE results corrected by self-constant and the correction equation. The NSE correction equation for instrumental method was established by analyzing the correlation of Hb results determined by HiCN method and instrumental method. Results The change constants of NSE in 72 neonates with serum hemolysis was（23.70 ±3.62） mg/g Hb. The relationship of concentration variation between Hb and NSE in neonatal bloodsample was ΔNSE_serum=23.17×ΔHb_HiCN （Y=23.17X,r²=0.812 2,P<0.001）. There was no statistical significance of NSE corrected results between self-constant group and correction equation group（P=0.541）. The linearity for Hb of instrumental method with HiCN was high（r²=0.992 4,P<0.001）. The correction equations of NSE in neonatal hemolytic serum were NSE_correction=NSE_measurement-23.17Hb_HiCN and NSE_correction=NSE_measurement-23.60Hb_instrument+13.22. Conclusions Hemolysis has a serious influence on the determination of NSE in serum. For neonatal hemolytic sera,it is critical to evaluate the severity of neonatal hypoxic ischemic encephalopathy by correcting NSE level.

Key words: Neuron-specific enolase, Hemolysis, Sample, Correction equation, Neonate

CLC Number:

R446.1

WANG Qiang, LU Xiaolan, WANG Guangrong, CAI Yanjuan, DU Qin, ZHANG Maoxin, ZHANG Guoyuan, WANG Dongsheng. Influence of neonatal blood sample hemolysis on the determination of serum neuron-specific enolase and establishment of correction equation[J]. Laboratory Medicine, 2019, 34(1): 42-46.

Figures/Tables 4

References 12

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