Laboratory Medicine ›› 2017, Vol. 32 ›› Issue (11): 985-989.DOI: 10.3969/j.issn.1673-8640.2017.011.010

• Orginal Article • Previous Articles     Next Articles

Drug resistance genes in carbapenem-resistant Enterobacteriaceae

HE Jianye1, CAO Zaiqiu2, DAI Zhifeng3   

  1. 1. Department of Clinical Laboratory,Children's Hospital of Kaifeng City,Kaifeng 475001,Henan,China
    2. Department of Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,Henan,China
    3. Department of Clinical Laboratory,Huaihe Hospital,Henan University,Kaifeng 475001,Henan,China
  • Received:2017-02-21 Online:2017-11-30 Published:2017-12-07

Abstract:

Objective To investigate the drug resistance genes in carbapenem-resistant Enterobacteriaceae isolated from the First Affiliated Hospital of Zhengzhou University. Methods The clinical isolates of Escherichia coli and Klebsiella pneumoniae were collected from June 2014 to December 2015,and they were screened by Vitek 2 Compact automatic bacterium identification system,according to the Clinical and Laboratory Standards Institute(CLSI) 2013. A total of 29 isolates being resistant to imipenem/meropenem were sorted out except repeated isolates. The encoding genes of KPC,NDM,VIM,SPM,GIM,IMP,OXA23,OXA24,OXA51 and OXA58 were analyzed by polymerase chain reaction(PCR). Results Among the 29 isolates,there were 11 isolates of KPC positive(10 isolates of Klebsiella pneumoniae and 1 isolate of Escherichia coli). A total of 7 isolates were NDM positive(3 isolates of Klebsiella pneumoniae and 4 isolates of Escherichia coli),and 2 isolates were identified as variant gene NDM-5. Conclusions Resistance to carbapenems is mainly due to the production of KPC and NDM in Enterobacteriaceae.

Key words: Carbapenems, Enterobacteriaceae, KPC, NDM-5, Three-dimensional structure

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