Abstract:

Objective To analyze Epstein-Barr virus （EBV） protein epitope polypeptide sequence and its human leukocyte antigen （HLA）genotypes with high frequency in Chinese. Methods A total of 23 polypeptides of EBV protein epitope were designed by NetMHC software. According to HLA phenotypes,HLA-A*2402,HLA-A*1101 and HLA-B*4001,polypeptides were classified into 3 groups. Peripheral blood mononuclear cells （PBMC） collected from 160 healthy subjects were stimulated with 3 groups of polypeptides. Interferon-gamma （IFN-γ） levels were determined by IFN-γ enzyme-linked immunosorbent assay （ELISA）. CD8,interleukin 17 （IL-17）,CD40L,tumor necrosis factor alpha （TNF-α）,CD45RA and Foxp3 expression levels were determined by flow cytometry. Results In 160 peripheral blood samples,61 cases were positive,of which 24 cases with HLA-A*2402 positive（15%）,34 cases were HLA-A*1101 positive（21%）,31 cases were HLA-B*4001 positive （19%）. The expression levels of CD8,IL-17,CD40L and TNF-α in peripheral blood in positive reaction group were higher than those in negative reaction group （P<0.05）,but the expression levels of CD45RA and Foxp3 had no statistical significance（P>0.05）. Conclusions In Chinese,HLA-A*1101 genotype is dominant in EBV protein epitope polypeptide sequence.

Key words: Epstein-Barr virus, Epitope, Human leukocyte antigen, Immune response, Interferon, CD8+T cells