Abstract:

Objective To investigate white blood cell（WBC） cell population data（CPD） in the diagnosis of dengue by UniCel DxH 800 automatic hematology analyzer,and to establish the optimal cut-off value.Methods A total of 159 healthy subjects,278 dengue patients and 106 suspected dengue patients（they had similar clinical symptoms with dengue patients,but their serological markers were negative） were enrolled. Whole blood count and WBC differential count were determined by DxH 800. Monocyte and lymphocyte CPD were analyzed. Receiver operating characteristic（ROC） curve was used to evaluate diagnosis efficiency. Results WBC and platelet（PLT） counts in dengue group were lower than those in suspected dengue and healthy control groups（P<0.05）,but the percentage of monocyte（MO%）was higher（P<0.05）,there was marked anisocytosis of monocytes and increased monocyte volume standard deviation（s-V-MO）（P<0.05）. Dengue indicator（MO%+s-V-MO） showed the optimal performance with an area under curve（AUC） of 0.99,when the cut-off value was 29.3,the sensitivity was 95.7%,and the specificity was 98.7%,for healthy control group. There was optimal performance with an AUC of 0.824,when the cut-off value was 34.8,the sensitivity was 78.3%,and the specificity was 75.2%,for suspected dengue group. Conclusions Dengue indicator is of significance in the diagnosis and differentiation of dengue.

Key words: Hematology analyzer, Dengue, Cell population data