In 2012, 313 S. aureus isolated from Shanghai Renji Hospital and 153 S. aureus isolated from Shanghai Children's Medical Center were collected and analyzed by multilocus sequence typing (MLST) and Staphylococcus protein A spa gene typing. In addition, staphylococcal cassette chromosome mec(SCCmec) typing was performed for methicillin-resistant Staphylococcus aureus(MRSA). Antimicrobial susceptibility test was detected by Kirby-Bauer method.

There were 313 isolates of S. aureus isolated from Shanghai Renji Hospital in 2012, mainly isolated from sputum, accounting for 42.49%, 173 isolates were MRSA, and the detection rate was 55.27%. The predominant type of MLST was ST5. The predominant type of spa gene typing was t002(90 isolates, 52.02%), and next type was t030(42 isolates, 24.28%). The predominant type of SCCmec typing was Ⅱ. There were 153 isolates of S. aureus isolated from Shanghai Children's Medical Center, mainly isolated from sputum, accounting for 62.09%, 33 isolates were identified as MRSA, and the detection rate was 21.57%. The predominant type of MLST was ST59. The predominant type of spa gene typing was t437(14 isolates, 42.42%), and next type was t002(5 isolates, 15.15%) and t316(4 isolates, 12.12%). The predominant type of SCCmec typing was Ⅳ. The antimicrobial susceptibility test showed that all the S. aureus isolates were 100% sensitive to vancomycin, norvancomycin, teicoplanin, linezolid and tetracycline, and drug resistance rate was obviously lower than others. The drug resistance rates of MRSA from Shanghai Children's Medical Center to ciprofloxacin, moxifloxacin, levofloxacin, clindamycin, rifampicin and gentamicin were significantly higher than those from Shanghai Renji Hospital(P<0.01).For cefazolin and erythromycin, there was no statistical significance(P>0.01).

The type of ST5-t002-Ⅱis the major epidemic MRSA type in Shanghai Renji Hospital, and ST59-t437-Ⅳ is the major epidemic MRSA type in Shanghai Children's Medical Center in 2012.Unlike methicillin-sensitive Staphylococcus aureus (MSSA), MRSA shows clustered clonal types, suggesting a nosocomial spread in hospital. The isolation rate of MRSA in S. aureus is high. Clinic should rationally use antimicrobial agents according to the results of drug susceptibility.