Primary investigation on in vitro bacteriostatic action of Amur-corktree to Staphylococcus aureus

doi:10.3969/j.issn.1673-8640.2015.09.005

Abstract

Abstract: Objective

To investigate in vitro bacteriostatic action of Amur-corktree to Staphylococcus aureus.

Methods

The minimum inhibitory concentrations (MIC) of Amur-corktree to 20 isolates of Staphylococcus aureus were determined by agar dilution method, normal dilution method and micro-dilution method.

Results

The MIC for inhibiting 90% bacteria (MIC90) of agar dilution method was 12.5 mg/mL, the MIC90 of normal dilution method was 25 mg/mL, and the MIC90 of micro-dilution method was 25 mg/mL.

Conclusions

It exists an obvious in vitro bacteriostatic action of Amur-corktree to Staphylococcus aureus.

Key words: Amur-corktree, Staphylococcus aureus, In vitro bacteriestasis

CLC Number:

R378.1

ZHAO Lingxu, WANG Lei. Primary investigation on in vitro bacteriostatic action of Amur-corktree to Staphylococcus aureus[J]. Laboratory Medicine, 2015, 30(9): 886-889.

Figures/Tables 2

References 14

[1]	张义虎,孙静. 黄柏的临床应用总述[J]. 中国医学创新,2010,7(3): 182-183.
[2]	李峰,贾彦竹. 黄柏的临床药理作用[J]. 中医药临床杂志,2004,16(2): 191.
[3]	王跃华,徐文俊,何俊蓉,等. 川黄柏离体培养及药用成分的抑菌试验研究[J]. 中国中药杂志, 2004,29(10): 1002-1003.
[4]	夏美玲,吕丽艳,刘野,等. 5种中草药体外抑菌实验的研究[J]. 齐齐哈尔医学院学报,2009,30(24):3024-3025.
[5]	张景皓,李艳红,赵虎. 五味子对鲍曼不动杆菌体外杀菌和抑菌作用的研究[J]. 检验医学,2014,29(6): 664-667.
[6]	王秀华,李仲兴,张新华,等. 五倍子等14种中药对112株表皮葡萄球菌的抗菌活性观察[J]. 解放军药学学报,2007,23(4): 275-277.
[7]	徐红云,刘春林,李宜铮,等. 伤口感染病原菌分布及耐药谱分析[J]. 中国微生态学杂志, 2013,25(11): 1313-1316.
[8]	胡仁静,严子禾,胡锡池. 金黄色葡萄球菌的耐药分析[J]. 职业与健康,2009,25(24): 2859-2860.
[9]	李娟,李晓东,杨丽霞,等. 单味中药体外抑菌活性的研究进展[J]. 中国实验方剂学杂志,2011,17(11): 283-286.
[10]	杨勇,叶小利,李学刚. 4种黄连生物碱的抑菌作用[J]. 时珍国医国药,2007,18(12): 3013-3014.
[11]	SEVERINAⅡ,MUNTYAN MS,LEWIS K,et al.Transfer of cationic antibacterial agents berberine, palmatine, and benzalkonium through bimolecular planar phospholipid film and Staphylococcus aureus membrane[J]. IUBMB Life,2001, 52(6): 321-324.
[12]	李波,朱维良,陈凯先. 小檗碱及其衍生物的研究进展[J]. 药学学报,2008,43(8): 773-787.
[13]	周树勤,姚芬,黄源春,等. 小檗碱对MRSA自溶酶系统作用的研究[J]. 中国感染与化疗杂志,2012,12(4): 310-312.
[14]	郭丽双,葛立男,宋宝辉,等. 中药体外抑菌实验检测方法的比较[J]. 中医学报,2011,27(10): 1207-1208.

菌株	孔号[药液浓度(g/mL)]
菌株	1(100)	2(50)	3(25)	4(12.5)	5(6.25)	6(3.125)	7(1.563)	8(0.781)	9(0.391)	10(0)	11(0)
1	0.010	0.012	-0.011	0.069	0.068	0.104	0.190	0.218	0.207	0.207	0.001
2	0.008	-0.009	0.008	0.082	0.095	0.133	0.183	0.222	0.202	0.227	0.003
3	0.018	0.015	0.012	0.010	0.091	0.174	0.216	0.183	0.219	0.187	0.002
4	0.018	0.019	0.013	0.009	0.086	0.122	0.119	0.202	0.169	0.190	0.003
5	0.020	0.018	0.015	0.017	0.115	0.145	0.144	0.206	0.182	0.190	0.000
6	0.017	0.016	0.014	0.011	0.075	0.163	0.157	0.156	0.206	0.202	0.000
7	0.015	0.010	0.012	0.093	0.082	0.124	0.136	0.157	0.180	0.184	0.005
8	-0.007	0.008	0.009	0.082	0.118	0.142	0.183	0.219	0.182	0.174	0.004
9	0.012	0.015	0.009	0.013	0.116	0.163	0.187	0.237	0.234	0.193	-0.002
10	0.013	0.018	0.008	0.015	0.116	0.146	0.156	0.205	0.246	0.191	-0.001
11	0.014	0.017	0.007	0.017	0.104	0.151	0.147	0.196	0.246	0.175	0.002
12	0.015	0.019	0.009	0.073	0.077	0.153	0.148	0.190	0.184	0.176	0.000
13	0.011	0.005	0.008	0.082	0.081	0.165	0.176	0.201	0.222	0.185	-0.002
14	0.009	0.013	0.018	0.012	0.091	0.146	0.195	0.202	0.192	0.192	0.003
15	0.008	0.010	0.012	0.010	0.105	0.175	0.182	0.180	0.185	0.186	0.001
16	-0.004	-0.008	0.008	0.091	0.102	0.123	0.145	0.196	0.212	0.192	0.000
17	0.012	0.017	0.015	0.092	0.092	0.157	0.146	0.182	0.197	0.192	0.002
18	0.013	0.016	0.011	0.097	0.072	0.163	0.150	0.191	0.186	0.185	-0.003
19	0.011	-0.007	0.016	0.091	0.113	0.170	0.152	0.182	0.203	0.179	0.002
20	0.005	0.008	0.012	0.101	0.112	0.161	0.172	0.179	0.196	0.201	0.003

菌株	孔号[药液浓度(g/mL)]
菌株	1(100)	2(50)	3(25)	4(12.5)	5(6.25)	6(3.125)	7(1.563)	8(0.781)	9(0.391)	10(0)	11(0)
1	0.010	0.012	-0.011	0.069	0.068	0.104	0.190	0.218	0.207	0.207	0.001
2	0.008	-0.009	0.008	0.082	0.095	0.133	0.183	0.222	0.202	0.227	0.003
3	0.018	0.015	0.012	0.010	0.091	0.174	0.216	0.183	0.219	0.187	0.002
4	0.018	0.019	0.013	0.009	0.086	0.122	0.119	0.202	0.169	0.190	0.003
5	0.020	0.018	0.015	0.017	0.115	0.145	0.144	0.206	0.182	0.190	0.000
6	0.017	0.016	0.014	0.011	0.075	0.163	0.157	0.156	0.206	0.202	0.000
7	0.015	0.010	0.012	0.093	0.082	0.124	0.136	0.157	0.180	0.184	0.005
8	-0.007	0.008	0.009	0.082	0.118	0.142	0.183	0.219	0.182	0.174	0.004
9	0.012	0.015	0.009	0.013	0.116	0.163	0.187	0.237	0.234	0.193	-0.002
10	0.013	0.018	0.008	0.015	0.116	0.146	0.156	0.205	0.246	0.191	-0.001
11	0.014	0.017	0.007	0.017	0.104	0.151	0.147	0.196	0.246	0.175	0.002
12	0.015	0.019	0.009	0.073	0.077	0.153	0.148	0.190	0.184	0.176	0.000
13	0.011	0.005	0.008	0.082	0.081	0.165	0.176	0.201	0.222	0.185	-0.002
14	0.009	0.013	0.018	0.012	0.091	0.146	0.195	0.202	0.192	0.192	0.003
15	0.008	0.010	0.012	0.010	0.105	0.175	0.182	0.180	0.185	0.186	0.001
16	-0.004	-0.008	0.008	0.091	0.102	0.123	0.145	0.196	0.212	0.192	0.000
17	0.012	0.017	0.015	0.092	0.092	0.157	0.146	0.182	0.197	0.192	0.002
18	0.013	0.016	0.011	0.097	0.072	0.163	0.150	0.191	0.186	0.185	-0.003
19	0.011	-0.007	0.016	0.091	0.113	0.170	0.152	0.182	0.203	0.179	0.002
20	0.005	0.008	0.012	0.101	0.112	0.161	0.172	0.179	0.196	0.201	0.003

[1]	FANG Yongmei, ZHANG Yan, XU Xueying, ZHONG Feng. Correlation of Staphylococcus aureus drug resistance with drug resistance genes and virulence genes [J]. Laboratory Medicine, 2023, 38(4): 357-361.
[2]	TANG Jin, PAN Yunqi, YANG Yumei, WU Qiong, WANG Jianqiang, LI Yungai, DI Jianzhong. Research progress of methicillin-resistant Staphylococcus aureus screening and decolonization to reduce surgical site infection [J]. Laboratory Medicine, 2022, 37(5): 489-492.
[3]	BAO Taoxiang, SONG Haoyue, YANG Han, HE Chunyan, SHU Wen, CHEN Wenjing, TANG Rong, LIU Qingzhong. Prevalence and genetic characteristic of Staphylococcus aureus with agr dysfunction [J]. Laboratory Medicine, 2021, 36(7): 743-748.
[4]	SUN Gang, SUN Hui, DU Yandan, LI Yinyan, LI Yingzhi, DONG Zhanzhu, TONG Lijun, PENG Bo. Study on the drug resistance and molecular epidemiological characteristics of MRSA in Hulunbuir [J]. Laboratory Medicine, 2021, 36(6): 600-603.
[5]	GUO Kunshan, WANG Shanmei, MA Bing, XU Junhong, ZHANG Jiangfeng, JING Nan, YAN Wenjuan, MA Qiong, LI Yi. Application of MALDI-TOF MS in methicillin-resistant Staphylococcus aureus rapid identification [J]. Laboratory Medicine, 2021, 36(2): 209-212.
[6]	ZHANG Li, SUN Linchun. Determination and drug resistance gene of methicillin-resistant Staphylococcus aureus isolated from adults in a hospital of Nanjing [J]. Laboratory Medicine, 2019, 34(3): 215-220.
[7]	ZHOU Shan, LIU Jiayun, MA Yueyun, ZHOU Ke, ZHOU Lei, HAO Xiaoke, XU Xiuli. Drug resistance and molecular epidemiology of Staphylococcus aureus with suppurative infection [J]. Laboratory Medicine, 2019, 34(2): 140-143.
[8]	YANG Shaoyou, HUANG Mengqi, ZENG Songfang, LIN Yuanshuang, FANG Guanghui. Surveillance and drug resistance of Gram-positive bacteria in Cangnan County [J]. Laboratory Medicine, 2019, 34(2): 148-151.
[9]	LU Hanwen, ZHOU Wanqing, ZHANG Zhifeng, SHEN Han. Comparison of phenotypic methods for β-lactamase detection in Staphylococcus aureus [J]. Laboratory Medicine, 2019, 34(2): 169-172.
[10]	CHEN Wenjing, HE Chunyan, YANG Han, ZHAO Huanqiang, CUI Zelin, TANG Rong, LIU Qingzhong. Drug resistance of methicillin-resistant Staphylococcus aureus to mupirocin,fusidic acid and retapamulin [J]. Laboratory Medicine, 2019, 34(1): 33-37.
[11]	YAO Dongting, HU Jun, ZHANG Xueqing, HU Xiaobo. Inhibitory activity of Chinese medicine coptischinensisfranch against Staphylococcus aureus in vitro [J]. Laboratory Medicine, 2017, 32(7): 577-581.
[12]	CHEN Ye, DONG Yefeng, DONG Deping. Molecular characteristics and drug resistance of Staphylococcus aureus isolated from patients with skin and soft tissue infections in Haian [J]. Laboratory Medicine, 2016, 31(10): 844-847.
[13]	WANG Junrui, WEI Changmei, TA La, CUI Jinghua, DU Xiaoli, HAN Yanqiu. Study on the diversity of in vitro invasion abilities of Staphylococcus aureus clinical isolates with different genotypes [J]. Laboratory Medicine, 2015, 30(7): 740-746.
[14]	LIANG Xiaoyi, ZHU Haiying, LIU Xiaomei, PAN Min. Analysis on the in vitro susceptibility test results from two groups of traditional Chinese medicine prescriptions for diabetic foot producing ESBLs Escherichia coli and MRSA [J]. Laboratory Medicine, 2015, 30(3): 261-264.
[15]	NIE Zhiyan, CHEN Xu, CHEN Hong, HAN Lizhong, NI Yuxing. Surveillance on nasal carriage rate and drug resistance of Staphylococcus aureus among 859 youngsters in Shanghai [J]. Laboratory Medicine, 2015, 30(2): 156-159.