Surveillance on nasal carriage rate and drug resistance of Staphylococcus aureus among 859 youngsters in Shanghai

doi:10.3969/j.issn.1673-8640.2015.02.013

Abstract

Abstract: Objective

To survey the nasal carriage rate and drug resistance of Staphylococcus aureus among 859 youngsters in Shanghai.

Methods

The nasal specimens were collected from 859 youngsters. The isolates were identified by Gram staining, ornithine decarboxylase test, deoxyribonuclease test, growth test on mannitol salt agar and plasma tube coagulase test. Antimicrobial susceptibility test was performed by disk diffusion method. Staphylococcal protein A (SPA) typing and Panton-Valentine leukocidin (PVL) gene were performed by polymerase chain reaction (PCR).

Results

The nasal carriage rate of Staphylococcus aureus was 7.0% (60 cases), no methicillin-resistant Staphylococcus aureus (MRSA) was detected, and no PVL toxicity factor was found in these isolates. There were 59 isolates with clone diversity, 1 isolate was spa gene negative, and 3 novel spa types were found.

Conclusions

The nasal carriage rate of Staphylococcus aureus is low, and no MRSA is found in these youngsters.

Key words: Staphylococcus aureus, Carriage rate, Youngster

CLC Number:

R378.1

NIE Zhiyan, CHEN Xu, CHEN Hong, HAN Lizhong, NI Yuxing. Surveillance on nasal carriage rate and drug resistance of Staphylococcus aureus among 859 youngsters in Shanghai[J]. Laboratory Medicine, 2015, 30(2): 156-159.

Figures/Tables 4

References 11

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抗菌药物	耐药 (株数)	中介 (株数)	敏感 (株数)	耐药率 (%)
青霉素	42	-	18	70
苯唑西林	0	1	59	0
头孢西丁	0	-	60	0
万古霉素	0	0	60	0
庆大霉素	3	0	57	5
卡那霉素	6	1	53	10
莫匹罗星	0	0	60	0

抗菌药物	耐药 (株数)	中介 (株数)	敏感 (株数)	耐药率 (%)
青霉素	42	-	18	70
苯唑西林	0	1	59	0
头孢西丁	0	-	60	0
万古霉素	0	0	60	0
庆大霉素	3	0	57	5
卡那霉素	6	1	53	10
莫匹罗星	0	0	60	0

spa分型	spa重复序列	株数	spa分型	spa重复序列	株数
t002	26-23-17-34-17-20-17-12-17-16	2	t688	26-23-17-34-17-16	1
t011	08-16-02-25-34-24-25	1	t701	11-10-21-17-34-24-34-22-25-25	3
t078	04-21-12-41-20-17-12-12-17	1	t758	14-44-12-17-23-23-18-17-17	1
t084	07-23-12-34-34-12-12-23-02-12-23	3	t774	07-23-12-34-34-12-12-12-23-02-12-23	1
t091	07-23-21-17-34-12-23-02-12-23	3	t954	26-23-17-34-17-17-16	1
t127	07-23-21-16-34-33-13	2	t1107	26-17-20-17-12-16	1
t148	07-23-12-21-12-17-20-17-12-12-17	2	t1376	07-12-21-17-13-13-34-34-33-13	3
t163	04-20-17-20-17-45-16-34	2	t1425	14-44-13-12-17-17	1
t164	07-06-17-21-34-34-22-34	1	t2186	07-23-12-21-17-17-34	1
t189	07-23-12-21-17-34	5	t3930	04-34-21-17-34-21-17-21-17-34-22-25	1
t213	07-23-12-21-24-33-22-17	1	t4258	15-12-41-20-17-12-12-17	2
t223	26-23-13-23-05-17-25-17-25-16-28	1	t4549	04-34-21-17-21-17-34-22-25	1
t338	15-21-16-02-25-17-24	1	t4897	07-23-12-21-17-20-17-12-12-17	1
t363	15-16-02-25-17-24	1	t5104	08-16-02-25-34-02-25-34-25	1
t437	04-20-17-20-17-25-34	1	new type 1	04-20-17-13-20-17-25-34	1
t548	26-23-17-34-17-20-17-12-16	4	new type 2	14-44-17-23-23-18-17-17	1
t571	08-16-02-25-02-25-34-25	2	new type 3	14-44-12-13-12-17-17-17	1
t616	04-34-17-32-17-23-24-24	3	NT	—	1
t645	14-44-13-12-17-23-18-17	1

spa分型	spa重复序列	株数	spa分型	spa重复序列	株数
t002	26-23-17-34-17-20-17-12-17-16	2	t688	26-23-17-34-17-16	1
t011	08-16-02-25-34-24-25	1	t701	11-10-21-17-34-24-34-22-25-25	3
t078	04-21-12-41-20-17-12-12-17	1	t758	14-44-12-17-23-23-18-17-17	1
t084	07-23-12-34-34-12-12-23-02-12-23	3	t774	07-23-12-34-34-12-12-12-23-02-12-23	1
t091	07-23-21-17-34-12-23-02-12-23	3	t954	26-23-17-34-17-17-16	1
t127	07-23-21-16-34-33-13	2	t1107	26-17-20-17-12-16	1
t148	07-23-12-21-12-17-20-17-12-12-17	2	t1376	07-12-21-17-13-13-34-34-33-13	3
t163	04-20-17-20-17-45-16-34	2	t1425	14-44-13-12-17-17	1
t164	07-06-17-21-34-34-22-34	1	t2186	07-23-12-21-17-17-34	1
t189	07-23-12-21-17-34	5	t3930	04-34-21-17-34-21-17-21-17-34-22-25	1
t213	07-23-12-21-24-33-22-17	1	t4258	15-12-41-20-17-12-12-17	2
t223	26-23-13-23-05-17-25-17-25-16-28	1	t4549	04-34-21-17-21-17-34-22-25	1
t338	15-21-16-02-25-17-24	1	t4897	07-23-12-21-17-20-17-12-12-17	1
t363	15-16-02-25-17-24	1	t5104	08-16-02-25-34-02-25-34-25	1
t437	04-20-17-20-17-25-34	1	new type 1	04-20-17-13-20-17-25-34	1
t548	26-23-17-34-17-20-17-12-16	4	new type 2	14-44-17-23-23-18-17-17	1
t571	08-16-02-25-02-25-34-25	2	new type 3	14-44-12-13-12-17-17-17	1
t616	04-34-17-32-17-23-24-24	3	NT	—	1
t645	14-44-13-12-17-23-18-17	1

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