Laboratory Medicine ›› 2015, Vol. 30 ›› Issue (10): 983-986.DOI: 10.3969/j.issn.1673-8640.2015.10.004

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Research on genotype and relationship of genotype with drug-resistant phenotypes from 266 Mycobacterium tuberculosis in Qingpu District

PENG Rong, FEI Fengying.   

  1. Department of Clinical Laboratory, Shanghai Qingpu District Central Hospital, Shanghai 201700, China
  • Received:2015-01-08 Online:2015-10-30 Published:2015-11-04

Abstract: Objective

To investigate the genotype and relationship of genotype with drug-resistant phenotypes of Mycobacterium tuberculosis(MTB)popular isolates in Qingpu, Shanghai.

Methods

The DNA of 266 MTB isolates were extracted and identified as Beijing genotype and non-Beijing genotype by multiplex polymerase chain reaction (PCR). Drug sensitivity tests of the 266 MTB isolates against streptomycin, isoniazid, rifampicin and ethambutol were performed by proportion method. The relationships of genotype with sex, age and drug-resistant phenotypes were analyzed by SPSS 17.0 statistical software, respectively.

Results

The 242 isolates (90.98%) were identified as Beijing genotype, while 24 isolates (9.02%) were identified as non-Beijing genotype. The overall drug resistance rate of Beijing genotype isolates was 23.96%, while multidrug-resistance rate was 9.91%.The overall drug resistance rate and multidrug-resistance rate were 29.16% and 8.83% as to non-Beijing genotype.

Conclusions

The MTB popular isolates of Qingpu, Shanghai might be Beijing genotype, but it exists significant difference between Beijing and non-Beijing genotypes for drug resistance.

Key words: Mycobacterium tuberculosis, Beijing genotype, Drug resistance

CLC Number: