Performance evaluation of Cobio Palio 300 urine biochemical analyzer for the determinations of 5 urinary micro-proteins

doi:10.3969/j.issn.1673-8640.2015.06.015

Abstract

Abstract: Objective

To evaluate the performance of Cobio Palio 300 urine automatic biochemical analyzer (Palio 300) for the determinations of 5 kinds of urinary micro-proteins.

Methods

By Palio 300 and Siemens BN-prospec, 120 urine samples were determined for urinary micro-albumin (mAlb), α₁ microglobulin (α₁-MG), β₂ microglobulin (β₂-MG), transferrin (TRF) and immunoglobulin G (IgG). The results were compared and analyzed statistically. In addition, the medium-value and high-value mixed urine was used for these items. Each sample was determined repeatedly for 20 times, and the coefficient of variation (CV) was calculated.

Results

Four items showed CV<6.00%, except β₂-MG CV=8.66%. The results were consistent with those of Siemens BN-prospec. The correlation coefficients (r) of 5 items were >0.9 (mAlb 0.953, α₁-MG 0.915, β₂-MG 0.927, TRF 0.918 and IgG 0.935, P<0.001).

Conclusions

Palio 300 has good repeatability, and its result is consistent with Siemens BN-prospec in the determinations of 5 urinary micro-proteins.

Key words: Micro-albumin, α1 microglobulin, β2 microglobulin, Transferrin, Immunoglobulin G, Urine, Cobio Palio 300 urine biochemical analyzer, Turbidimetric immunoassay

CLC Number:

R446.12

HUANG Yan, XU Xiaorong, ZHANG Jue. Performance evaluation of Cobio Palio 300 urine biochemical analyzer for the determinations of 5 urinary micro-proteins[J]. Laboratory Medicine, 2015, 30(6): 603-606.

Figures/Tables 8

项目	Palio 300	BN-prospec
mAlb	010100	153976C
β₂-MG	020200	42273
α₁-MG	020200	42128
TRF	020200	153444A
IgG	010100	153079K
尿液多项蛋白定标液	12/001	083696C
尿液蛋白质质控液	11/003	084638G(低值)
	11/003	084741E(中值)
	11/003	084840D(高值)

项目	中值		高值		性能要求[CV(%)]
项目	$x ?$ ±s(mg/L)	CV(%)		$x ?$ ±s(mg/L)	CV(%)
mAlb	26.59±0.60	2.27	81.50±1.55	1.90	5
β₂-MG	33.96±2.94	8.66	101.39±3.33	3.28	10
α₁M	0.37±0.022	5.88	1.30±0.071	5.48	10
TRF	7.98±0.39	4.83	26.23±0.89	3.41	5
IGg	57.29±2.39	4.18	176.66±4.84	2.74	5

项目	中值		高值		性能要求[CV(%)]
项目	$x ?$ ±s(mg/L)	CV(%)		$x ?$ ±s(mg/L)	CV(%)
mAlb	26.59±0.60	2.27	81.50±1.55	1.90	5
β₂-MG	33.96±2.94	8.66	101.39±3.33	3.28	10
α₁M	0.37±0.022	5.88	1.30±0.071	5.48	10
TRF	7.98±0.39	4.83	26.23±0.89	3.41	5
IGg	57.29±2.39	4.18	176.66±4.84	2.74	5

项目	r值	b值	a值	线性范围 (mg/L)	P值
mAlb	0.953	0.965	2.195	2.30119.70	<0.001
β₂-MG	0.927	0.862	0.059	0.171.84	<0.001
α₁-MG	0.915	0.905	4.679	10.24104.13	<0.001
TRF	0.918	1.145	-0.076	2.7151.16	<0.001
IgG	0.935	1.104	17.052	4.40290.90	<0.001

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