Application of MALDI-TOF MS technology in clinical microbial determination

doi:10.3969/j.issn.1673-8640.2015.02.002

Abstract

Abstract:

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is one kind of soft ionization mass spectrometry, which is suitable for the analysis of bio-macromolecule, such as protein. It can be used for microbial identification by mapping the microbial protein mass spectra. Since MALDI-TOF MS technology is well-known for its rapidity, accuracy, high sensitivity and high throughput, it has been applied in bacterium and fungus identifications. MALDI-TOF MS also has a good performance in the field of scientific research, including resistance analysis, bacterium typing and virulence study,and there is still a large space for development. In this paper, we reviewed the application of MALDI-TOF MS technology in microbial identification and scientific research.

Key words: Matrix-assisted laser desorption/ionization-time of flight mass spectrometry, Microbiology, Determination, Scientific research

CLC Number:

R446.5

DAI Yingxin, LI Min. Application of MALDI-TOF MS technology in clinical microbial determination[J]. Laboratory Medicine, 2015, 30(2): 102-107.

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