Abstract: Objective　To investigate the interference of lipemia on automatic hematology analyzers.   Methods　Normal fresh whole blood samples were determined for complete blood cell（CBC） count by automatic hematology analyzer，and the plasma were replaced by same volume of fat emulsion after centrifugal separation to make artificial lipemia [blood triglyceride（TG）＞4.0 mmol/L]. These samples were tested for CBC by the same method before they were added lipid to estimate which parameters can be affected by lipemia.  Results　When the TG＞4.0 mmol/L，lipemia could make pseudo increase of hemoglobin（Hb），mean corpuscular hemoglobin（MCH） and mean corpuscular hemoglobin concentration（MCHC）（P＜0.05）. When the TG＞14.45 mmol/L，fat particles can make pseudo increase of the white blood cell（WBC） count with statistical significance（P＜0.05）.  Conclusions　When TG＞4.0 mmol/L，Hb，MCH and MCHC need to be corrected. The higher TG concentration is，the greater interference of Hb，MCH and MCHC are. The lower Hb concentration is，the greater interference of TG is. When TG＞14.45 mml/L，WBC needs to be corrected.

Key words: Lipemia interference, Blood cell analysis