Study on the killing activity of DC-CIK cells pulsed with gastric cancer cells of heat shock against cancer cells

›› 2012, Vol. 27 ›› Issue (10): 849-853.

Study on the killing activity of DC-CIK cells pulsed with gastric cancer cells of heat shock against cancer cells

1. Ningxia Medical University，Ningxia Yinchuan 750004，China；2. Medical Research Center，Ningxia Medical University General Hospital，Ningxia Yinchuan 750004，China

Received:2012-05-14 Revised:2012-07-05 Online:2012-10-30 Published:2012-10-12

Abstract

Abstract: Objective　To study the killing effect of dendritic cells (DC) and cytokine-induced killer (CIK) cells pulsed with specific antigens against gastric cancer cells. Methods　Peripheral blood mononuclear cells（PBMC）were isolated from healthy subjects. DC and CIK cells were induced by different cytokines. Gastric cell line MKN-28 lysates of heat shock were used as antigen and pulsed DC，which co-cultured with CIK cells. The phenotypes of DC and CIK cell membranes were determined by flow cytometry，and the killing activity of CIK against MKN-28 was determined by (MTS). Results　Compared with CIK，the co-cultured DC-CIK presented a significantly higher proliferation. The numbers of CD3⁺CD8⁺ and CD3⁺CD56⁺ cells increased，and the killing effect was enhanced. The cytotoxic activity to MKN-28 of DC-CIK pulsed with MKN-28 antigen(Ag-DC-CIK) group was （57.96±2.23）% ，which was more strong than（38.02±2.06）% of DC-CIK pulsed without MKN-28 antigen group and（29.78±1.84）% of CIK group，respectively [the ratio of effect to target =20.0∶1]. Conclusions　Heat shock tumor-lysate-pulsed DC can strengthen the proliferation and killing activity of CIK against gastric cancer cells.

Key words: Cytokine-induced killer cell, Dendritic cell, Heat shock, Gastric cancer

ZHANG Chenglei 1，CHEN Yaoping 2，YANG Baozhen 2，WU Ruofen 2，WANG Yanbai 1. Study on the killing activity of DC-CIK cells pulsed with gastric cancer cells of heat shock against cancer cells[J]. , 2012, 27(10): 849-853.

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