Abstract: Objective　To evaluate the efficiency of imported anti-hepatitis C virus（HCV） enzyme-linked immunosorbent assay（ELISA） reagent in blood screening test and analyze the correlations among imported ELISA reagent，domestic ELISA reagent and recombinant immunoblot assay（RIBA）. Methods　A total of 56 anti-HCV positive specimens tested by imported ELISA reagent were retested by 2 domestic ELISA reagents，and the confirmatory test RIBA was also carried out. Results　Among 56 positive specimens with imported anti-HCV ELISA reagent，the confirmatory test RIBA showed that the 12 specimens were confirmed to be positive ，18 specimens were indeterminate and 26 specimens were negative. Of the 9 specimens which were positive with 3 ELISA reagents，7 specimens were confirmed to be positive，1 specimen was indeterminate and 1 specimen was negative. Conclusions　The false positivity with anti-HCV ELISA reagent is obvious，and there would be some different results with different anti-HCV ELISA reagents. For ensuring the blood safety，the imported ELISA reagent and the domestic ELISA reagent should be used simultaneously in anti-HCV screening test.

Key words: Anti-hepatitis C virus antibody, Enzyme-linked immunosorbent assay, Recombinant immunoblot assay