Characteristics of TCR profile in multiple sclerosis patients based on high-throughput sequencing technology

doi:10.3969/j.issn.1673-8640.2025.05.002

Abstract

Abstract:

Objective To investigate the profile characteristics of complementary determining region 3 （CDR3） of T cell receptor （TCR） β chain in patients with multiple sclerosis （MS）. Methods The T cell immune repertoire sequencing data of 11 female MS patients （MS group） and 12 healthy females （healthy control group） were downloaded from the ImmuneAccess database. The TCR profiles of all research subjects were analyzed systematically，which included CDR3 frequency distribution，CDR3 length distribution，TRβ V，TRβ D and TRβ J usage frequencies，V-J combination frequency and insertion/deletion length distribution of Vdels，Jdels，D5dels，D3dels，n1ins and n2ins sites. The diversity of TCR profiles based on D50 index，Shannon index，Simpson index and Inverse simpson index was calculated. Results The diversity of TCRβ chain CDR3 in MS group was higher than that in healthy control group （P<0.01）. Compared with healthy control group，the frequency of TCRβ chain CDR3 nucleotide lengths at 57，60，63 and 66 nt in MS group was increased （P<0.05），while the CASSLRETQYF sequence in TCRβ chain CDR3 amino acid sequence was decreased （P<0.05）. There was no statistical significance in the length distribution of insertion deletion in the In and Out sequences，the utilization frequency of 20 amino acids in the 6th and 7th amino acids of TCRβ chain CDR3 and the utilization frequency of hydrophilic，neutral and hydrophobic amino acids between MS group and healthy control group（P>0.05）. There was statistical significance in the utilization frequency of TRβ V，TRβ D and TRβ J gene subfamilies between MS group and healthy control group（P<0.05）. Conclusions The TCR profile of MS group is different from that of healthy control group，which provides a reference for the diagnosis and treatment of MS patients.

Key words: T cell receptor, Complementary determining region 3, Multiple sclerosis, Profile

CLC Number:

R446.62

CHEN Xiaoyan, MO Qianer, HUANG Fangying, HU Zipeng, YANG Shanshan, NONG Jingjing, YANG Xueli. Characteristics of TCR profile in multiple sclerosis patients based on high-throughput sequencing technology[J]. Laboratory Medicine, 2025, 40(5): 421-427.

Figures/Tables 6

References 30

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