Correlation and clinical significance of serum lncRNA THRIL and miR-495-3p expressions with common intestinal bacterial species in children with inflammatory bowel disease

doi:10.3969/j.issn.1673-8640.2025.06.009

Abstract

Abstract:

Objective To investigate serum long non-coding RNA（lncRNA） tumor necrosis factor-related heterogeneous ribonucleoprotein particle（THRIL） and miR-495-3p expressions in children with inflammatory bowel disease（IBD） and their correlation with common intestinal bacterial species，and to provide a reference for the clinical diagnosis of IBD. Methods From June 2021 to May 2023，109 children with IBD admitted to Zhengzhou Yihe Hospital were enrolled as study group，and another 109 healthy children were enrolled as control group. The common intestinal bacterial species in the fecal samples of enrolled children were identified by culture methods. The expression levels of serum lncRNA THRIL and miR-495-3p were determined by reverse transcription polymerase chain reaction（PCR）. Using ENCORI database，the binding site between lncRNA THRIL and miR-495-3p was predicted. The relevant clinical data of study group and control group were collected，and the differences between the 2 groups were compared. Spearman method was used to analyze the correlation between the expression levels of serum lncRNA THRIL and miR-495-3p and the number of common intestinal bacterial species. Logistic regression analysis was used to analyze the related factors for the occurrence of IBD. The diagnostic value of serum lncRNA THRIL and miR-495-3p for IBD was analyzed by receiver operating characteristic（ROC） curve. Results The proportion of patients with a history of intestinal diseases and unhealthy dietary habits in study group was higher than that in control group（P<0.01）. There was statistical significance in the colony numbers of Escherichia coli，Bifidobacterium，Lactobacillus，Enterococcus and yeast between the 2 groups（P<0.001）. The relative expression level of serum lncRNA THRIL in study group was higher than that in control group（P<0.001），while the relative expression level of miR-495-3p was lower than that in control group（P<0.001），and there were complementary sites in the nucleic acid sequences of the 2 indicators. Serum lncRNA THRIL relative expression level was positively correlated with the colony numbers of Escherichia coli，Enterococcus and yeast（r=0.543，0.551 and 0.703，P<0.001），and it was negatively correlated with the colony numbers of Bifidobacterium and Lactobacillus（r=-0.561 and -0.627，P<0.001）. The miR-495-3p was on the contrary. History of gastrointestinal diseases [odds ratio（OR）=1.772，P=0.029]，unhealthy dietary habits（OR=2.048，P=0.010），increased lncRNA THRIL（OR=12.117，P=0.001） and decreased miR-495-3p（OR=0.665，P=0.043） were risk factors for the occurrence of IBD. The combined determination of serum lncRNA THRIL and miR-495-3p was superior to the single determinations（P<0.001）. Conclusions The relative expression levels of serum lncRNA THRIL and miR-495-3p are related to the common intestinal bacterial species of patients with IBD. The combined determination has a high diagnostic value for IBD.

Key words: Long non-coding RNA, Tumor necrosis factor-related heterogeneous ribonucleoprotein particle, MicroRNA-495-3p, Common intestinal bacterial sepecies, Inflammatory bowel disease, Children

CLC Number:

R446.7

YAN Hua, GAO Dahan, LIU Dongfeng. Correlation and clinical significance of serum lncRNA THRIL and miR-495-3p expressions with common intestinal bacterial species in children with inflammatory bowel disease[J]. Laboratory Medicine, 2025, 40(6): 565-570.

Figures/Tables 8

References 23

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引物名称	正向引物（5'~3'）	反向引物（5'~3'）
lncRNA THRIL	AACTTCACAGGAACACTACACAAGA	TAGGCAACAGAGAGCAAGACTTCATC
miR-495-3p	GCGGAAACAAACATGGTGCA	CAGTGCGTGTCGTGGAGT
GAPDH	GCACCCTCAAGGCTGAGAAC	TGGTGAAGACGCCAGTGGA
U6	ATTGGAACGATACAGAGAAGATT	GGAACGCTTCACGAATTTG

引物名称	正向引物（5'~3'）	反向引物（5'~3'）
lncRNA THRIL	AACTTCACAGGAACACTACACAAGA	TAGGCAACAGAGAGCAAGACTTCATC
miR-495-3p	GCGGAAACAAACATGGTGCA	CAGTGCGTGTCGTGGAGT
GAPDH	GCACCCTCAAGGCTGAGAAC	TGGTGAAGACGCCAGTGGA
U6	ATTGGAACGATACAGAGAAGATT	GGAACGCTTCACGAATTTG

组别	年龄		性别		体重指数		营养不良
组别	<8岁	≥8岁	男	女	<22 kg·m^-2	≥22 kg·m^-2	是	否
对照组	53.95（41/76）	47.89（68/142）	46.02（52/113）	54.29（57/105）	47.00（47/100）	52.54（62/118）	52.73（87/165）	41.51（22/53）
研究组	46.05（35/76）	52.11（74/142）	53.98（61/113）	45.71（48/105）	53.00（53/100）	47.46（56/118）	47.27（78/165）	58.49（31/53）
χ²值	0.727		1.488		0.665		2.019
P值	0.394		0.222		0.415		0.155
组别	家族IBD疾病史			肠胃疾病史			不健康饮食习惯
组别	有	无		有	无		有	无
对照组	42.62（26/61）	52.87（83/157）		37.93（44/116）	63.73（65/102）		38.61（39/101）	59.83（70/117）
研究组	57.38（35/61）	47.13（74/157）		62.07（72/116）	36.27（37/102）		61.39（62/101）	40.17（47/117）
χ²值	1.844			14.445			9.759
P值	0.175			<0.001			0.002

组别	年龄		性别		体重指数		营养不良
组别	<8岁	≥8岁	男	女	<22 kg·m^-2	≥22 kg·m^-2	是	否
对照组	53.95（41/76）	47.89（68/142）	46.02（52/113）	54.29（57/105）	47.00（47/100）	52.54（62/118）	52.73（87/165）	41.51（22/53）
研究组	46.05（35/76）	52.11（74/142）	53.98（61/113）	45.71（48/105）	53.00（53/100）	47.46（56/118）	47.27（78/165）	58.49（31/53）
χ²值	0.727		1.488		0.665		2.019
P值	0.394		0.222		0.415		0.155
组别	家族IBD疾病史			肠胃疾病史			不健康饮食习惯
组别	有	无		有	无		有	无
对照组	42.62（26/61）	52.87（83/157）		37.93（44/116）	63.73（65/102）		38.61（39/101）	59.83（70/117）
研究组	57.38（35/61）	47.13（74/157）		62.07（72/116）	36.27（37/102）		61.39（62/101）	40.17（47/117）
χ²值	1.844			14.445			9.759
P值	0.175			<0.001			0.002

组别	例数	大肠埃希菌/ （lgCFU·g^-1）	双歧杆菌/ （lgCFU·g^-1）	乳酸杆菌/ （lgCFU·g^-1）	肠球菌/ （lgCFU·g^-1）	葡萄球菌/ （lgCFU·g^-1）	酵母菌/ （lgCFU·g^-1）
对照组	109	5.72±1.03	6.41±1.21	5.26±1.08	5.08±0.92	4.36±0.79	2.03±0.35
研究组	109	6.33±1.27	4.61±0.96	3.84±0.72	7.06±1.52	4.53±0.86	2.35±0.49
t值		3.895	12.167	11.422	11.635	1.520	5.548
P值		<0.001	<0.001	<0.001	<0.001	0.130	<0.001