Research progress on a gene editing and molecular diagnostic system CRISPR-Cas13a

doi:10.3969/j.issn.1673-8640.2022.01.020

Abstract

Abstract:

Clustered regularly interspaced short palindromic repeats（CRISPR）-CRISPR-associated protein（Cas） system is a powerful tool for gene editing. Compared with Cas9,Cas13a targeted multiple gene transcription products and regulated gene function expression,filling Cas9 with defects such as editing and missing effect only limited to DNA level. Moreover,using the characteristics of Cas13a targeting RNA,the system has been successfully transformed into the next generation of nucleic acid diagnostic tools. In this review,the latest research progress of CRISPR-Cas13 system in the field of gene editing and molecular diagnosis is summarized,and the application prospect of this system has prospected.

Key words: Clustered regularly interspaced short palindromic repeat, Clustered regularly interspaced short palindromic repeat-associated protein 13a, Incidental cleavage, Gene editing, Specific high-sensitivity enzymatic reporter unlocking

CLC Number:

R446.1

CHEN Jialing, HUANG Yinger, DENG Hao, WU Hongfeng, ZHANG Jing, ZHAO Yijun, LUO Jiahao, HAO Wenbo. Research progress on a gene editing and molecular diagnostic system CRISPR-Cas13a[J]. Laboratory Medicine, 2022, 37(1): 97-100.

Figures/Tables 2

References 23

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