Progress of IPAI experimental diagnosis

doi:10.3969/j.issn.1673-8640.2021.04.022

Abstract

Abstract:

The diagnostic performance of invasive pulmonary Aspergillus infection（IPAI） determinations were evaluated in terms of etiology（smear microscopy,culture）,immunohistochemistry,molecular biology,13 kinds biomarkers of Aspergillus and combined determination. The positive rate of immunohistochemistry was 87.5%. The sensitivity of smear microscopy was 87.7%,and the specificity was 72.1%. The positive rate of culture was <61%. Among these specimens,sputum accounted for 12%,bronchoalveolar lavage fluid（BALF） accounted for 40%-50%,and blood accounted for <5%. The sensitivity and the specificity of molecular biology were 50%-100% and 75%-89%,respectively. The sensitivity of biomarkers was 61%-100%,and the specificity was 77%-95%. The sensitivity of combined determination was 73%-94%,and the specificity was 0.81%-99.4%. The sensitivities and specificities of these technologies are different,the determations of biomarkers have the characteristics of rapidity,high sensitivity and high specificity,and the combined determination is of great significance.

Key words: Invasive pulmonary Aspergillus infection, Aspergillus, Determination, Sensitivity, Specificity, Biomarker

CLC Number:

R446.1

GAO Dongtian, LIU Lihua, SHEN Aihua, SUN Yin. Progress of IPAI experimental diagnosis[J]. Laboratory Medicine, 2021, 36(4): 453-461.

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