Determination and quantitation of blood human papillomavirus DNA in healthy subjects

doi:10.3969/j.issn.1673-8640.2021.11.006

Abstract

Abstract:

Objective To study blood human papillomavirus（HPV） DNA load of healthy subjects,and to evaluate its potential clinical significance. Methods The blood samples from 207 healthy subjects were collected,and genomic DNA was extracted. GP5+/GP6+ primers were used for quantitative polymerase chain reaction（PCR）. Simultaneously,140-150 bp specific fragment of HPV L1 gene was sequenced to classify genotypes. Results HPV DNA was determined in 14（6.8%） blood donors in the 207 healthy subjects. The 7 cases were single type HPV infection,and 7 cases were mixed infection. The viral load of all the positive samples was 12.4-39.3 copies/mL. Conclusions There is a low load of HPV DNA in the blood of some healthy subjects,and the blood may be a new way of HPV transmission. It is not excluded that some healthy blood donors have potential HPV infection in the reproductive tract or other parts,or have a history of HPV infection.

Key words: Human papillomavirus, Blood, Quantitative polymerase chain reaction, Genotyping

CLC Number:

R373.9

LIU Hongqian, SONG Chaohui, LIANG Qiaomi. Determination and quantitation of blood human papillomavirus DNA in healthy subjects[J]. Laboratory Medicine, 2021, 36(11): 1121-1124.

Figures/Tables 1

References 25

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献血者编号	HPV感染型别	HPV载量/（拷贝/mL）
1	16、18	15.3
2	16、32、45、66	16.8
3	70	25.1
4	16	20.2
5	33	20.1
6	16、18	16.8
7	16、18	17.8
8	82	18.3
9	51	39.3
10	16	23.7
11	16、18	12.4
12	16、18	16.4
13	16	16.8
14	16、51、59	39.2

献血者编号	HPV感染型别	HPV载量/（拷贝/mL）
1	16、18	15.3
2	16、32、45、66	16.8
3	70	25.1
4	16	20.2
5	33	20.1
6	16、18	16.8
7	16、18	17.8
8	82	18.3
9	51	39.3
10	16	23.7
11	16、18	12.4
12	16、18	16.4
13	16	16.8
14	16、51、59	39.2