Laboratory Medicine ›› 2021, Vol. 36 ›› Issue (10): 1060-1063.DOI: 10.3969/j.issn.1673-8640.2021.010.015
Previous Articles Next Articles
Received:
2020-06-28
Online:
2021-10-30
Published:
2021-11-08
CLC Number:
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2021.010.015
保存 温度 | 例数 | 基线水平 | 保存时间 | χ2值 | P值 | |||
---|---|---|---|---|---|---|---|---|
1 d | 3 d | 5 d | 7 d | |||||
常温 | 50 | 0.86(0.78~0.94) | 0.87(0.74~0.91) | 0.86(0.74~0.94) | 0.85(0.76~0.92) | 0.86(0.79~0.95) | 5.00 | 0.287 |
4 ℃ | 50 | 0.86(0.78~0.94) | 0.85(0.78~0.94) | 0.86(0.78~0.94) | 0.85(0.78~0.92) | 8.44 | 0.077 | |
-20 ℃ | 50 | 0.53(0.49~0.63)* | 0.44(0.40~0.51)* | 0.40(0.35~0.48)* | 0.37(0.31~0.46)* | 200.00 | <0.001 | |
-80 ℃ | 50 | 0.52(0.49~0.57)* | 0.43(0.40~0.47)* | 0.40(0.37~0.43)* | 0.37(0.34~0.40)* | 198.41 | <0.001 | |
χ2值 | 147.61 | 148.95 | 148.96 | 149.01 | ||||
P值 | <0.001 | <0.001 | <0.001 | <0.001 |
保存 温度 | 例数 | 基线水平 | 保存时间 | χ2值 | P值 | |||
---|---|---|---|---|---|---|---|---|
1 d | 3 d | 5 d | 7 d | |||||
常温 | 50 | 0.86(0.78~0.94) | 0.87(0.74~0.91) | 0.86(0.74~0.94) | 0.85(0.76~0.92) | 0.86(0.79~0.95) | 5.00 | 0.287 |
4 ℃ | 50 | 0.86(0.78~0.94) | 0.85(0.78~0.94) | 0.86(0.78~0.94) | 0.85(0.78~0.92) | 8.44 | 0.077 | |
-20 ℃ | 50 | 0.53(0.49~0.63)* | 0.44(0.40~0.51)* | 0.40(0.35~0.48)* | 0.37(0.31~0.46)* | 200.00 | <0.001 | |
-80 ℃ | 50 | 0.52(0.49~0.57)* | 0.43(0.40~0.47)* | 0.40(0.37~0.43)* | 0.37(0.34~0.40)* | 198.41 | <0.001 | |
χ2值 | 147.61 | 148.95 | 148.96 | 149.01 | ||||
P值 | <0.001 | <0.001 | <0.001 | <0.001 |
保存温度 | 例数 | 基线/[例(%)] | 保存时间/[例(%)] | |||
---|---|---|---|---|---|---|
1 d | 3 d | 5 d | 7 d | |||
常温 | 50 | 9(18) | 9(18) | 9(18) | 9(18) | 9(18) |
4 ℃ | 50 | 9(18) | 9(18) | 9(18) | 9(18) | |
-20 ℃ | 50 | 6(12) | 3(6) | 3(6) | 3(6) | |
-80 ℃ | 50 | 5(10) | 3(6) | 3(6) | 3(6) |
保存温度 | 例数 | 基线/[例(%)] | 保存时间/[例(%)] | |||
---|---|---|---|---|---|---|
1 d | 3 d | 5 d | 7 d | |||
常温 | 50 | 9(18) | 9(18) | 9(18) | 9(18) | 9(18) |
4 ℃ | 50 | 9(18) | 9(18) | 9(18) | 9(18) | |
-20 ℃ | 50 | 6(12) | 3(6) | 3(6) | 3(6) | |
-80 ℃ | 50 | 5(10) | 3(6) | 3(6) | 3(6) |
保存 温度 | 例数 | 基线水平 | 冻融次数 | |||
---|---|---|---|---|---|---|
1次 | 2次 | 3次 | 4次 | |||
常温 | 61 | 1.90(0.82~5.22) | ||||
-20 ℃ | 61 | 1.20(0.48~3.60)* | 1.14(0.43~3.39)* | 1.10(0.41~3.13)* | 1.02(0.38~2.61)* | |
-80 ℃ | 61 | 1.20(0.48~3.60)* | 1.13(0.41~3.38)* | 1.10(0.42~3.15)* | 1.01(0.37~2.59)* | |
Z值 | 0.000 | -1.826 | -0.365 | -1.841 | ||
P值 | 1.000 | 0.068 | 0.715 | 0.066 |
保存 温度 | 例数 | 基线水平 | 冻融次数 | |||
---|---|---|---|---|---|---|
1次 | 2次 | 3次 | 4次 | |||
常温 | 61 | 1.90(0.82~5.22) | ||||
-20 ℃ | 61 | 1.20(0.48~3.60)* | 1.14(0.43~3.39)* | 1.10(0.41~3.13)* | 1.02(0.38~2.61)* | |
-80 ℃ | 61 | 1.20(0.48~3.60)* | 1.13(0.41~3.38)* | 1.10(0.42~3.15)* | 1.01(0.37~2.59)* | |
Z值 | 0.000 | -1.826 | -0.365 | -1.841 | ||
P值 | 1.000 | 0.068 | 0.715 | 0.066 |
基线S/CO值 | 例数 | -20 ℃ | -80 ℃ | |||||||
---|---|---|---|---|---|---|---|---|---|---|
冻融1次 | 冻融2次 | 冻融3次 | 冻融4次 | 冻融1次 | 冻融2次 | 冻融3次 | 冻融4次 | |||
<1 | 15 | 41.77 | 48.68 | 50.65 | 54.60 | 44.93 | 49.12 | 50.45 | 54.50 | |
1~<2 | 17 | 36.84 | 40.13 | 42.11 | 46.05 | 36.91 | 40.28 | 43.05 | 46.10 | |
2~<5 | 14 | 30.92 | 34.94 | 40.16 | 49.80 | 30.90 | 34.89 | 42.47 | 49.78 | |
≥5 | 15 | 9.99 | 12.04 | 15.03 | 18.96 | 8.94 | 12.01 | 15.71 | 19.63 |
基线S/CO值 | 例数 | -20 ℃ | -80 ℃ | |||||||
---|---|---|---|---|---|---|---|---|---|---|
冻融1次 | 冻融2次 | 冻融3次 | 冻融4次 | 冻融1次 | 冻融2次 | 冻融3次 | 冻融4次 | |||
<1 | 15 | 41.77 | 48.68 | 50.65 | 54.60 | 44.93 | 49.12 | 50.45 | 54.50 | |
1~<2 | 17 | 36.84 | 40.13 | 42.11 | 46.05 | 36.91 | 40.28 | 43.05 | 46.10 | |
2~<5 | 14 | 30.92 | 34.94 | 40.16 | 49.80 | 30.90 | 34.89 | 42.47 | 49.78 | |
≥5 | 15 | 9.99 | 12.04 | 15.03 | 18.96 | 8.94 | 12.01 | 15.71 | 19.63 |
[1] |
ZEMLIN A E. Errors in the extra-analytical phases of clinical chemistry laboratory testing[J]. Indian J Clin Biochem, 2018, 33(2):154-162.
DOI URL |
[2] |
CARRARO P, PLEBANI M. Errors in a stat laboratory:types and frequencies 10 years later[J]. Clin Chem, 2007, 53(7):1338-1342.
DOI URL |
[3] | SIMUNDIC A M, LIPPI G. Preanalytical phase-a continuous challenge for laboratory professionals[J]. Biochem Med(Zagreb), 2012, 22(2):145-149. |
[4] | DAGUR P K, MCCOY JP Jr. Collection, storage, and preparation of human blood cells[J]. Curr Protoc Cytom, 2015, 73:5.1.1-5.1.16. |
[5] | GISLEFOSS R E, LAURITZEN M, LANGSETH H, et al. Effect of multiple freeze-thaw cycles on selected biochemical serum components[J]. Clin Chem Lab Med, 2017, 55(7):967-973. |
[6] |
GUNASEKARA F I, RICHARDSON K, CARTER K, et al. Fixed effects analysis of repeated measures data[J]. Int J Epidemiol, 2014, 43(1):264-269.
DOI URL |
[7] | CUHADAR S, KOSEOGLU M, ATAY A, et al. The effect of storage time and freeze-thaw cycles on the stability of serum samples[J]. Biochem Med(Zagreb), 2013, 23(1):70-77. |
[8] | 余国庆, 孙红, 周丽玲, 等. 血清标本保存条件及反复冻融对胃蛋白酶原检测结果影响[J]. 中外医学研究, 2018, 16(7):73-75. |
[9] |
HILLEBRAND J J, HEIJBOER A C, ENDERT E. Effects of repeated freeze-thaw cycles on endocrine parameters in plasma and serum[J]. Ann Clin Biochem, 2017, 54(2):289-292.
DOI URL |
[10] | 司学众, 卜颖, 贾楠, 等. 血清样本反复冻融对甲状腺功能相关实验室指标检测结果的影响[J]. 中国卫生检验杂志, 2010, 20(10):2524-2525. |
[11] | 尹云弟, 李玲, 孙玉娟, 等. 不同储存条件和时间对HCV RNA检测结果的影响[J]. 中国输血杂志, 2018, 31(6):613-616. |
[1] | FAN Lieying. Research status and application prospects of biomarkers for membranous nephropathy [J]. Laboratory Medicine, 2023, 38(12): 1111-1114. |
[2] | SUN Lincheng, LI Jianfeng, CHENG Weili, JI Panyun. Roles of anti-PLA2R IgG4 antibody and PLA2R IgG4/IgG ratio in primary membranous nephropathy [J]. Laboratory Medicine, 2023, 38(12): 1115-1120. |
[3] | LIU Qingyang, YUAN Jianming, XIA Jinjun, JIANG Fengying, WANG Qiubo, WANG Xiaoming. CXCL9 as a potential diagnostic marker of rheumatoid arthritis based on GEO database and experimental verification [J]. Laboratory Medicine, 2023, 38(12): 1121-1129. |
[4] | YANG Xiao, LI Enling, WU Lixia, DAI Yingxin, WANG Zhiqing, HUANG Hao, ZHENG Bing. Influence of cytoplasmic antinuclear antibody on indirect immunofluorescence assay based antineutrophil cytoplasmic antibody determination [J]. Laboratory Medicine, 2023, 38(12): 1135-1140. |
[5] | XIANG Jin, LIU Aiping, HU Yao, WU Zhiyuan, CAO Guojun, GUAN Ming. ANA profiles in COVID-19 patients and influence of serum heat-inactivation on ANA determination [J]. Laboratory Medicine, 2023, 38(12): 1141-1146. |
[6] | CHENG Yu, XU Zhen, LU Liu, DING Menglei, YU Shanshan, ZONG Ming, FAN Lieying. Expression and risk factors of anti-ACE-2 antibody in serum of patients with COVID-19 [J]. Laboratory Medicine, 2023, 38(12): 1147-1152. |
[7] | . [J]. Laboratory Medicine, 2023, 38(12): 1153-1156. |
[8] | . [J]. Laboratory Medicine, 2023, 38(12): 1157-1159. |
[9] | WANG Hongling, LIU Mengna, BAI Ping, LIAO Huanjin. Risk factors of relapse following allogeneic hematopoietic stem cell transplantation in patients with acute myeloid leukemia [J]. Laboratory Medicine, 2023, 38(12): 1160-1166. |
[10] | YANG Xilan, WANG Jinyu, LIU Shuyu, ZHAN Yiyang, LIU Jing, JIA Jian. Correlation between hyperlipidemia and endothelial microparticles in elderly patients from Gulou Community in Nanjing [J]. Laboratory Medicine, 2023, 38(12): 1167-1172. |
[11] | DAI Fangfang, LU Xinxin, YU Yanhua, CHEN Ming, SUN Guizhen. Analysis of Mycobacterium infection in HIV/AIDS patients [J]. Laboratory Medicine, 2023, 38(12): 1173-1176. |
[12] | GU Yu, LIANG Xiaoyan, MA Shenghui, TONG Na, CHENG Mingyan, YAN Zejun. Expression and near-term prognostic predictive value of plasma biomarkers in chronic kidney disease patients with thromboembolism [J]. Laboratory Medicine, 2023, 38(12): 1177-1182. |
[13] | . [J]. Laboratory Medicine, 2023, 38(12): 1183-1185. |
[14] | . [J]. Laboratory Medicine, 2023, 38(12): 1186-1190. |
[15] | . [J]. Laboratory Medicine, 2023, 38(12): 1191-1194. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||