LncRNA SNHG1 promoting the growth of gastric cancer through binding to miR-145

doi:10.3969/j.issn.1673-8640.2020.10.023

Abstract

Abstract:

Objective To investigate small nucleolar RNA host gene 1（SNHG1） promoting the growth of gastric cancer through binding to microRNA-145（miR-145）. Methods Kaplan-Meier Plotter database analyzed the correlation of SNHG1 with gastric cancer overall survival. Bioinformatics analysis and real-time fluorescence quantitation polymerase chain reaction（qRT-PCR） were used to investigate the expression level and the expression relationship of SNHG1 and miR-145 in gastric cancer. The influence of overexpression and knock-down of SNHG1 on the growth of gastric cancer cell was determined by CCK8 assay. Luciferase reporter gene assay studied the interaction between SNHG1 and miR-145. Western blot was used to determined the change of CTNNB1 and MYC protein with the transfection of SNHG1,miR-145 and SNHG1 with miR-145. Results The overall survival of patients with low SNHG1 expression was longer than that of patients with high SNHG1 expression（P=0.015）. Compared to adjacent tissues,the expression level of SNHG1 in gastric cancer tissues was increased（P<0.05）. The overexpression of SNHG1 promoted the growth of gastric cancer cells,while knock-down SNHG1 inhibited cell growth. Luciferase reporter assay showed that SNHG1 could bind to miR-145 effectively. The miR-145 down-regulated the expressions of CTNNB1 and MYC proteins,SNHG1 promoted the expressions of CTNNB1 and MYC proteins,and SNHG1 inhibited the down-regulation of CTNNB1 and MYC proteins by miR-145. Conclusions The over-expressed SNHG1 in gastric cancer binding to miR-145 enhances the expression of CTNNB1 and MYC proteins that could promote gastric cancer growth.

Key words: Small nucleolar RNA host gene 1, MicroRNA-145, Cell growth, Gastric cancer

CLC Number:

R446.7

ZHOU Fengli, CHU Yimin, LI Ji, YANG Daming, PENG Haixia, XU Ying. LncRNA SNHG1 promoting the growth of gastric cancer through binding to miR-145[J]. Laboratory Medicine, 2020, 35(10): 1062-1069.

Figures/Tables 5

References 33

[1]	CHEN W,ZHENG R,BAADE P D,et al.Cancer statistics in China,2015[J]. CA Cancer J Clin,2016,66(2):115-132.
[2]	HAO N B,HE Y F,LI X Q,et al.The role of miRNA and lncRNA in gastric cancer[J]. Oncotarget,2017,8(46):81572-81582.
[3]	ZHANG X,LI M,CHEN S,et al. Endoscopic screening in Asian countries is associated with reduced gastric cancer mortality:a Meta-analysis and systematic review[J]. Gastroenterology,2018,155(2):347-354.e9.
[4]	HARROW J,FRANKISH A,GONZALEZ J M,et al.GENCODE:the reference human genome annotation for the ENCODE project[J]. Genome Res,2012,22(9):1760-1774.
[5]	CHEN G,QIU C,ZHANG Q,et al.Genome-wide analysis of human SNPs at long intergenic noncoding RNAs[J]. Hum Mutat,2013,34(2):338-344.
[6]	HUARTE M.The emerging role of lncRNAs in cancer[J]. Nat Med,2015,21(11):1253-1261.
[7]	曹季军,王金湖,申娴娟,等. lncRNA CCAT2在胃癌患者血清中的表达及其临床诊断意义[J]. 检验医学,2019,34(9):775-779.
[8]	明心亮,刘雪芳,朱满,等. 循环游离核酸在肝细胞肝癌临床诊治中的应用进展[J]. 检验医学,2019,34(7):667-671.
[9]	SUN J J,CHEN G Y,XIE Z T.MicroRNA-361-5p inhibits cancer cell growth by targeting CXCR6 in hepatocellular carcinoma[J]. Cell Physiol Biochem,2016,38(2):777-785.
[10]	WANG Y,CHEN J,LIN Z,et al.Role of deregulated microRNAs in non-small cell lung cancer progression using fresh-frozen and formalin-fixed,paraffin-embedded samples[J]. Oncol Let,2016,11(1):801-808.
[11]	XUAN Y,YANG H,ZHAO L,et al.MicroRNAs in colorectal cancer:small molecules with big functions[J]. Cancer Lett,2015,360(2):89-105.
[12]	王燕忠,丁丽敏,陈一瑞,等. miRNA-21-5p对食管癌的诊断价值及其与STAT3的相关性研究[J]. 检验医学,2020,35(6):531-534.
[13]	WANG Q,LI Q,ZHOU P,et al.Upregulation of the long non-coding RNA SNHG1 predicts poor prognosis,promotes cell proliferation and invasion,and reduces apoptosis in glioma[J]. Biomed Pharmacother,2017,91:906-911.
[14]	GUO W,HUANG J,LEI P,et al.LncRNA SNHG1 promoted HGC-27 cell growth and migration via the miR-140/ADAM10 axis[J]. Int J Biol Macromol,2019,122:817-823.
[15]	XU M,CHEN X,LIN K,et al.The long noncoding RNA SNHG1 regulates colorectal cancer cell growth through interactions with EZH2 and miR-154-5p[J]. Mol Cancer,2018,17(1):141.
[16]	LI Z,LU Q,ZHU D,et al.Lnc-SNHG1 may promote the progression of non-small cell lung cancer by acting as a sponge of miR-497[J]. Biochem Biophys Res Commun,2018,506(3):632-640.
[17]	HUANG L,JIANG X,WANG Z,et al.Small nucleolar RNA host gene 1:a new biomarker and therapeutic target for cancers[J]. Pathol Res Pract,2018,214(9):1247-1252.
[18]	HU Y,MA Z,HE Y,et al.LncRNA-SNHG1 contributes to gastric cancer cell proliferation by regulating DNMT1[J]. Biochem Biophys Res Commun,2017,491(4):926-931.
[19]	LEI C,DU F,SUN L,et al.miR-143 and miR-145 inhibit gastric cancer cell migration and metastasis by suppressing MYO6[J]. Cell Death Dis,2017,8(10):e3101.
[20]	WANG J,SUN Z,YAN S,et al.Effect of miR145 on gastric cancer cells[J]. Mol Med Rep,2019,19(5):3403-3410.
[21]	褚以忞,彭海霞,李吉,等. 微小RNA-145调控c-myc表达对胃癌的影响[J]. 上海医学,2014,37(11):953-956.
[22]	TIAN T,QIU R,QIU X.SNHG1 promotes cell proliferation by acting as a sponge of miR-145 in colorectal cancer[J]. Oncotarget,2018,9(2):2128-2139.
[23]	CHU Y M,PENG H X,XU Y,et al.MicroRNA-1254 inhibits the migration of colon adenocarcinoma cells by targeting PSMD10[J]. J Dig Dis,2017,18(3):169-178.
[24]	BRAY F,FERLAY J,SOERJOMATARAM I,et al.Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2018,68(6):394-424.
[25]	ZHAO Y,QIN Z S,FENG Y,et al.Long non-coding RNA(lncRNA) small nucleolar RNA host gene 1(SNHG1) promote cell proliferation in colorectal cancer by affecting P53[J]. Eur Rev Med Pharmacol Sci,2018,22(4):976-984.
[26]	ZHANG H,ZHOU D,YING M,et al.Expression of long non-coding RNA(lncRNA) small nucleolar RNA host gene 1(SNHG1) exacerbates hepatocellular carcinoma through suppressing miR-195[J]. Med Sci Monit,2016,22:4820-4829.
[27]	ZHANG H Y,YANG W,ZHENG F S,et al.Long non-coding RNA SNHG1 regulates zinc finger E-box binding homeobox 1 expression by interacting with TAp63 and promotes cell metastasis and invasion in lung squamous cell carcinoma[J]. Biomed Pharmacother,2017,90:650-658.
[28]	褚以忞,彭海霞,李吉,等. miR-145在胃黏膜癌变中的表达及其意义[J]. 胃肠病学,2013,18(1):45-47.
[29]	LI J,LI X,WU L,et al.miR-145 inhibits glutamine metabolism through c-myc/GLS1 pathways in ovarian cancer cells[J]. Cell Biol Int,2019,43(8):921-930.
[30]	SACHDEVA M,ZHU S,WU F,et al.p53 represses c-myc through induction of the tumor suppressor miR-145[J]. Proc Natl Acad Sci U S A,2009,106(9):3207-3212.
[31]	DU W,LI D,GUO X,et al.Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC[J]. Artif Cells Nanomed Biotechnol,2019,47(1):4120-4130.
[32]	SONG X,XIN N,WANG W,et al.Wnt/β-catenin,an oncogenic pathway targeted by H. pylori in gastric carcinogenesis[J]. Oncotarget,2015,6(34):35579-35588.
[33]	SCHOLZ B A,SUMIDA N,DE LIMA C D M,et al. WNT signaling and AHCTF1 promote oncogenic MYC expression through super-enhancer-mediated gene gating[J]. Nat Genet,2019,51(12):1723-1731.

肿瘤	位置/注释	基因	r值	P 值
TCGA-STAD	ENSG00000255717.2	hsa-mir-103-1	0.564	9.01×10^-8
TCGA-STAD	ENSG00000255717.2	hsa-mir-106b	0.477	1.01×10^-5
TCGA-STAD	ENSG00000255717.2	hsa-mir-107	0.579	3.51×10^-8
TCGA-STAD	ENSG00000255717.2	hsa-mir-128-1	0.486	6.47×10^-6
TCGA-STAD	ENSG00000255717.2	hsa-mir-128-2	0.474	1.14×10^-5
TCGA-STAD	ENSG00000255717.2	hsa-mir-139	–0.427	9.07×10^-5
TCGA-STAD	ENSG00000255717.2	hsa-mir-145	–0.421	1.17×10^-4
TCGA-STAD	ENSG00000255717.2	hsa-mir-151	0.539	3.89×10^-7
TCGA-STAD	ENSG00000255717.2	hsa-mir-15b	0.435	6.42×10^-5

肿瘤	位置/注释	基因	r值	P 值
TCGA-STAD	ENSG00000255717.2	hsa-mir-103-1	0.564	9.01×10^-8
TCGA-STAD	ENSG00000255717.2	hsa-mir-106b	0.477	1.01×10^-5
TCGA-STAD	ENSG00000255717.2	hsa-mir-107	0.579	3.51×10^-8
TCGA-STAD	ENSG00000255717.2	hsa-mir-128-1	0.486	6.47×10^-6
TCGA-STAD	ENSG00000255717.2	hsa-mir-128-2	0.474	1.14×10^-5
TCGA-STAD	ENSG00000255717.2	hsa-mir-139	–0.427	9.07×10^-5
TCGA-STAD	ENSG00000255717.2	hsa-mir-145	–0.421	1.17×10^-4
TCGA-STAD	ENSG00000255717.2	hsa-mir-151	0.539	3.89×10^-7
TCGA-STAD	ENSG00000255717.2	hsa-mir-15b	0.435	6.42×10^-5