Abstract:

Objective To investigate the combined determination of serum microRNA-598-3p （miR-598-3p） and carbohydrate antigen 153 （CA153）in the diagnosis of breast carcinoma. Methods A total of 100 breast carcinoma patients,45 benign breast disease controls and 40 healthy controls in Taizhou Central Hospital from January 2013 to September 2014. The level of serum miR-598-3p was determined by real-time fluorescence quantitation polymerase chain reaction （PCR）. The level of serum CA153 was determined by chemiluminescence. Receiver operating characteristic （ROC） curve was used to evaluate the sensitivities and specificities of serum miR-598-3p and CA153 single and combined determinations for diagnosing breast carcinoma. Results Compared to healthy control group,serum miR-598-3p was down-regulated,and serum CA153 was up-regulated in breast carcinoma group （P<0.01）. There was no correlation between serum miR-598-3p level and different TNM stages of breast carcinoma （P>0.05）. ROC curve showed that the sensitivities of serum miR-598-3p and CA153 were 95.0% and 66.0%,and the specificities were 85.0% and 65.0%,respectively. The sensitivity and specificity of the combined determination of serum miR-598-3p and CA153 were 96.0% and 70.0%. Conclusions MiR-598-3p shows a good sensitivity and specificity for diagnosing breast carcinoma,and the combined determination of serum miR-598-3p and CA153 could improve diagnostic efficiency.

Key words: MicroRNA-598-3p, Real-time fluorescence quantitation polymerase chain reaction, Breast carcinoma