Abstract:

Objective To analyze the differences and comparability of 25-hydroxyvitamin D [25 （OH） D] determination results in peripheral blood and venous blood,and to provide a reference for the homogeneity of 25（OH）D results determined by the 2 specimen types. Methods The levels of 25 （OH） D of peripheral blood and venous blood were determined by enzyme-linked immunosorbent assay. Linear regression analysis was used to analyze the correlation and bias of 25（OH）D determination results in the 2 specimen types. Results The levels of 25（OH）D of peripheral blood were lower than those of venous blood（P<0.01）. The ratio of peripheral blood 25（OH）D/ venous blood 25（OH）D was positively correlated with the ratio of peripheral blood hematocrit（HCT）/ venous blood HCT（r=0.634,P<0.01）. There was a correlation for the levels of 25（OH）D between peripheral blood and venous blood（r=0.995）. The bias of 25 （OH）D levels between the 2 specimen types was in allowed error provided by kits. Conclusions The levels of 25（OH）D of peripheral blood are lower than those of venous blood,and their results are comparable. Peripheral blood can be used to determine 25（OH）D levels.

Key words: 25-Hydroxyvitamin D, Bias, Peripheral blood, Venous blood