Abstract:

Objective To investigate the significance of the serum cytokeratin 18（CK-18） and liver fibrosis indexes level in non-alcoholic fatty liver disease （NAFLD）. Methods A total of 581 NAFLD patients and 186 healthy controls （healthy control group） were enrolled. Ultrasonic inspection was carried out to evaluate in NAFLD patients. Enzyme-linked immunosorbent assay （ELISA） was used to detect serum of CK-18 and chemiluminescence method was used to detect serum of procollagen type Ⅲ（PCⅢ）, collagen type Ⅳ （CⅣ）, laminin （LN）, hyaluronic acid（HA）. Results Compared with healthy controls,the light NAFLD、the moderate NAFLD、the severe NAFLD of the CK-18 （ng/L）、PC III（μg/L）、C IV（μg/L）、LN（μg/L）、HA（μg/L）respectively was：9.9±3.89,112.55±27.54,62.6±18.04,99.78±28.06,82.17±17.75;13.29±5,127.18±30.34,68.08±20.12,111.95±24.96,90.52±19.15;15.37±5.98,143.51±48.85,93.35±24.3,133.42±21.15,141.99±26.65;3.88±2.8,95.95±18.43,57±22.81,84.44±18.2,68.35±11.95. CK-18、PC III 、C IV 、LN and HA for the diagnosis of NAFLD area under the ROC curve respectively 0.927,0.771,0.793,0.808,0.717;Tangent point were 8.6,54.35,100.9,102.34,96.05; The sensitivity and specificity of CK -18 0.773 and 0.99,PC III was 0.747 and 0.77,C IV was 0.823 and 0.66,LN was 0.663 and 0.663,HA was 0.497 and 0.95. Conclusions As the degree of the NAFLD is aggravating,the serum of CK-18 、PC III、C IV、LN and HA level also gradually rise,is important for clinical diagnosis and monitoring. So in clinical diagnosis it has a certain value,18 CK - best diagnostic value and can be used as an independent predictor of NAFLD.

Key words: Non-alcoholic fatty liver disease, Cytokeratin-18, Liver fibrosis indexes, Fatty liver infiltration grade