Klebsiella pneumoniae was determined by VITEK-32 automatic microorganism analyzer, which was produced by France BioMerieux Company. A total of 627 samples were used for Klebsiella pneumoniae determination. Drug sensitivity test of Klebsiella pneumoniae was performed by disk diffusion (K-B ) method. Standard disk diffusion method suggested by the Clinical and Laboratory Standards Institute (CLSI) was employed to conduct the extended-spectrum beta lactamase (ESBL) test.

A total of 213 Klebsiella pneumoniae positive samples were determined from the 627 samples. The positive rate was 33.97%. The highest prevalence of Klebsiella pneumoniae positive samples was observed in the ward of Internal Medicine Department (66.67%), followed by Department of Obstetrics and Gynecology (43.84%) and Treatment Room (43.08%).The resistance rates of Klebsiella pneumoniae to ampicillin and amoxicillin were 96.70% and 99.73%, respectively. A total of 34.27% (73/213) of the determined Klebsiella pneumoniae was ESBL-produced Klebsiella pneumoniae.

The pollution of Klebsiella pneumoniae is common in medical environment with high resistance rate. In order to control the nosocomial infection of Klebsiella pneumoniae, hospital environment disinfection and inspection for Klebsiella pneumoniae pollution are needed to be further strengthened.