Laboratory diagnosis on three cases of imported plasmodium ovale malaria infection

doi:10.3969/j.issn.1673-8640.2014.08.016

Abstract

Abstract: Objective To analyze 3 cases of imported malaria infection, in order to reduce the misdiagnosis and missed diagnosis of ovale malaria infection. Methods By morphological test, nested polymerase chain reaction (PCR) and sequence analysis were used to confirm the 3 cases of imported malaria infection. Results The morphological test results showed no plasmodium (laboratory initial inspection), plasmodium ovale mixed infection with plasmodium falciparum and plasmodium ovale. However, plasmodium ovale, plasmodium ovale mixed infection with plasmodium falciparum and plasmodium ovale mixed infection with plasmodium vivax were detected by nested PCR by follow. Conclusions The morphological technique training and the application of molecular biology techniques should be strengthen for imported plasmodium ovale malaria infection, and can reduce the misdiagnosis and missed diagnosis of imported plasmodium ovale malaria infection.

Key words: Plasmodium ovale, Nested polymerase chain reaction, Sequence analysis, Mixed infection, Laboratory diagnosis

CLC Number:

R382.3

TIAN Bin, DUAN Jihui, XU Mingzhong, ZHANG Bing, LIAO Yu, SHEN Xiaojun, WEN Lan. Laboratory diagnosis on three cases of imported plasmodium ovale malaria infection[J]. , 2014, 29(8): 851-855.

References

[1] Craig CF. New varieties and species of malaria plasmodia[J]. J Parasitology, 1914, 1(2): 85-94.
[2] Stephens JWW. A new malaria parasite of man[J]. Ann Trop Med Parasitol, 1922, 87(14): 383-388.
[3] 张进顺,李薇,孙新,等.诊断医学寄生虫学[M]. 第5版. 北京:人民卫生出版社,2010:109-138.
[4] 汤林华,王汝波,王建军,等.输入性疟疾的诊治与管理[M].上海:上海科学技术出版社,2010:22-28.
[5] 张进顺,高兴政.临床寄生虫检验学[M]. 北京:人民卫生出版社,2009:103-109.
[6] Snounou G, Viryakosol S, Zhu XP, et al. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction[J]. Mol Biochem Parasitol, 1993,61(2):315-320.
[7] Snounou G, Viriyakosol S, Jarra W, et al. Identification of the four human malaria parasite species in field samples bythe polymerase chain reaction and detection of high prevalence of mixed infections[J]. Mol Biochem Parasitol, 1993,58(2): 283-292.
[8] Singh B, Bobogare A, Cox-Singh J, et al. A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies[J]. Am J Trop Med Hyg, 1999, 60(4): 687-692.
[9] Carme B, Kenmogne D, Copin N, et al. Plasmodium prevalence and parasitic burden in blood donors of Brazzaville, Congo[J]. Ann Soc Belg Med Trop, 1993,73(3):179-187.
[10] Gbakima AA. Inland valley swamp rice development: malaria, schistosomiasis, onchocerciasis in south central Sierra Leone[J]. Public Health, 1994,108(2):149-157.
[11] May J, Mockenhaupt FP, Ademowo OG, et al. High rate of mixed and subpatent malarial infections in southwest Nigeria[J]. Am J Trop Med Hyg, 1999,61(2):339-343.
[12] Gutman J, Guarner J. Pediatric malaria: 8-year case series in Atlanta, Georgia, and review of the literature[J]. J Travel Med, 2010,17(5):334-338.
[13] Rantala AM, Taylor SM, Trottman PA, et al. Comparison of real-time PCR and microscopy for malaria parasite detection in Malawian pregnant women[J]. Malar J, 2010,9:269.
[14] Ndao M, Bandyayera E, Kokoskin E, et al. Comparison of blood smear, antigen detection, and nested-PCR methods for screening refugees from regions where malaria is endemic after a malaria outbreak in Quebec, Canada [J]. J Clin Microbiol, 2004,42(6):2694-2700.
[15] 师永霞,黄吉城,苏锦坤,等.1例国内罕见的输入性卵形疟的实验室检测[J]. 中国人兽共患病学报, 2011,27(10):914-917.
[16] 段绩辉,王郭清,张湘君,等. 发病不稳定地区疟疾误诊原因分析[J]. 中国热带医学, 2007,7(7):1111-1112.
[17] Collins WE, Jeffery GM. Plasmodium ovale: parasite and disease[J]. Clin Microbiol Rev, 2005,18(3): 570-581.
[18] 王晨,陈和平,岳丹琪,等.上海地区人群中部分稀有血型筛选[J].检验医学,2007,22(5):583-584.
[19] Purnomo, Solihin A, Gomez-Saladin E, et al. Rare quadruple malaria infection in Irian Jaya Indonesia[J]. J Parasitol, 1999,85(3):574-579.