Study on influence of mitochondrial M2 protein on dendritic cells before and after silencing suppressor of cytokine signaling 1

Abstract

Abstract: Objective To study the effects of M2 protein on peripheral blood mononuclear cells(PBMC)-derived dendritic cell(DC) after silencing SOCS1. Methods: DC were generated from donor-derived PBMC and treated with SOCS-1 siRNA. DC were stimulated by various concentrations of M2 protein at different times CD83 and CD86 of DC was performed by flow cytometry（FCM）.The levels of interleukin-10(IL-10) and IL-12 in culture supernatant of DC were measured by enzyme linked immunosorbent assay (ELISA). Results The expression of CD83 and CD86, the levels of IL-10 and IL-12 of DC under stimulation of M2 protein at 70 μg/ml after 24 h, at 35 μg/ml after 24 and 48 h were all significantly higher than those in the control group（P<0.05）. After silencing SOCS1 by siRNA of DC in the presence of various concentrations of M2 at different times, the expression of CD83 and CD86, the levels of IL-12 were all increased significantly than those in the M2 group（P<0.05）. However, there were not any significant difference of IL-10 levels of DC between the two groups（P>0.05）. Conclusions The results indicated that DC after stimulation of M2 protein at high concentration had increased capacity to activate Th1 subset proliferation, maturation and antigen presentation. The functions was further enhanced after silencing SOCS1 of DC in the presence of M2, which maybe contribute to the broken of self-tolerance.

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