Clinical application evaluation of the FAST plaque TB assay for rapid detection of Mycobacterium tuberculosis

Abstract

Abstract: Objective　To evaluate the efficacy of FAST plaque TB assay for the direct detection of Mycobacterium tuberculosis in sputum specimens. Methods　A total of 530 sputum specimens were detected by FAST plaque TB assay，auramine O stain smear microscopy and a rapid culture assay. Results of a rapid culture assay were as the reference standards for evaluation. Results　Of the 530 sputum samples，there were 270 sputum samples positive by the rapid culture assay，including FAST plaque TB assay detected 220 positive. In the 260 negative sputum samples by the rapid culture assay，there were 20 positive by FAST plaque TB assay. The sensitivity，specificity，positive and negative predictive values of FAST plaque TB assay were 81.6%，92.3%，91.6% and 82.8%.The sensitivity，specificity，positive and negative predictive values of the combined determination of FAST plaque TB assay and smear microscopy were 88.9%，80.8%，82.8% and 87.5%. Conclusions　FAST plaque TB assay has certain high sensitivity and specificity ，as well as fast，safe and other advantages. The assay shows a high clinical significance especially for smear-negative tuberculosis patients.

QING Keqin;HAN Min;YUE Jun . Clinical application evaluation of the FAST plaque TB assay for rapid detection of Mycobacterium tuberculosis[J]. , 2012, 27(9): 760-763.

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