Abstract: Objective　To evaluate the methodology of determining blood manganese by inductively coupled plasma atomic emission spectrometry（ICP-AES），and apply the method in daily detection work. Methods　The whole blood samples were treated with nitric acid，and the concentration of the nitric acid was 1 mol/L. The supernatant fluid was collected. The concentrations of the whole blood manganese were determined by ICP-AES under the condition of 279.544 nm in 1 027 healthy children. The precision，recovery rate，sensitivity and anti-interference ability were evaluated，and the normal reference range was definited. Results　The within-run coefficient of variation（CV） was 6.2%-6.6%，the between-run CV was 7.1%-7.6%，and the total CV was 7.2%-7.8%. The relative standard deviation（RSD） was 1.66%-2.36%. The standardized recovery rate was 95.0%-96.9%. The linear equation was Y＝7 035.6X+759.02，and the correlation coefficient of the whole blood manganese was r＝0.999 7. The interference elements，K，Na，Cl，Ca and Mg（1∶〖KG-*2/5〗1，25 mg/L） and Fe（2 mg/L） were added into the blank solution and the standard reserve liquid of 100 μg/L manganese. The concentration of manganese had no obvious change. The normal reference range of whole blood manganese in 1 027 healthy children was（4.89±2.67） μg/L. Conclusions　ICP-AES for determining whole blood manganese has no obvious system error. This method is accurate and reliable，and can be applied as a conventional way to detect whole blood manganese.

Key words: Manganese, Whole blood, Inductively coupled plasma atomic emission spectrometry, Methodology evaluation