Abstract: Objective　To investigate the significance of BV Blue method in the diagnosis of bacterial vaginosis (BV), and provide rapid and reliable methods for diagnosing BV in clinic.   Methods　BV Blue method and Amsel method were used to determine 560 samples (<30 years old group, 31-40 years old group, 41-50 years old group and >50 years old group)， and the results were analyzed comparatively. Results　The positive rate of BV Blue method for diagnosing BV was 20.89%, and the positive rate of Amsel method was 24.82%. There was no significant difference （P>0.05）. The sensitivity， specificity and accuracy of BV Blue method for diagnosing BV was 97.86%， 77.79% and 92.85%, respectively. BV positive rate increased with age， and the highest rate was 28% when the age was >50 years old. Conclusions　BV Blue method is simple and rapid, and has high sensitivity and accuracy. It suitable for the rapid diagnosis of BV.

Key words: Bacterial vaginosis, Vaginal secretion, Amsel method, BV Blue method