Application evaluation of the real-time fluorescence quantitative PCR in the rapid identification of MRSA

Abstract

Abstract: Objective　To evaluate the application significance of the real-time fluorescence quantitative polymerase chain reaction（PCR） in the rapid identification of methicillin-resistant Staphylococcus aureus（MRSA）. Methods　A total of 85 Staphylococcus aureus strains were isolated from clinical samples，and MRSA and methicillin-sensitive Staphylococcus aureus（MSSA） were detected and differentiated by cefoxitin disk diffusion method and conventional PCR amplification of mecA gene. These strains were also detected by the real-time fluorescence quantitative PCR. The accordance of the real-time fluorescence quantitative PCR with conventional methods was evaluated. Results　According to the results of the cefoxitin disk diffusion method and conventional PCR amplification of mecA gene，45 out of 85 strains were MRSA，and 40 outof 85 strains were MSSA. The real-time fluorescence quantitative PCR had an excellent accordance（100%） with those methods. Conclusions　The real-time fluorescence quantitative PCR has an excellent accordance with conventional methods for detecting MRSA，and it has the advantages of ease of performance and costing short turn-around time. As a rapid identification method，the real-time fluorescence quantitative PCR can identify MRSA accurately，which is helpful for the clinical therapy of MRSA infections and for the control of MRSA transmission in hospitals.

Key words: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Real-time fluorescence quantitative polymerase chain reaction, Rapid identification

CHEN Xu 1，XIAO Shuzhen 1，DONG Danfeng 1，YANG Haihui 2，LI Shengxiang 3，NI Yuxing 1，HAN Lizhong 1. Application evaluation of the real-time fluorescence quantitative PCR in the rapid identification of MRSA[J]. , 2012, 27(12): 1035-1039.

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